FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569495
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19890.3492567976891808No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCCC17350.30465587933168864No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15620.27427808848190066No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12680.22265340345393728No Hit
CTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAG12520.21984389678574878No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11210.1968410609399556No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10770.18911491760243726No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10720.18823694676862834No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10220.17945723843053935No Hit
GACTATTACTGTTTGATTTGGCCAAACCATGTAGGGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAG10190.178930455930254No Hit
GTAATAGTCAGCCTCATCTTCAGACTGGAGCCCGGAAATGAGTAAAATCC10130.1778768909296833No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10040.17629654342882728No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9780.171731095093021No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9410.16523411092283516No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9220.16189782175436132No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9210.16172222758759955No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9070.15926390925293463No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC9070.15926390925293463No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9050.15891272091941105No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8370.14697231757961002No Hit
GTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTG8240.14468959341170684No Hit
GTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAG7770.1364366675739032No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7630.13397834923923826No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7600.13345156673895295No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7500.13169562507133514No Hit
ATGTAGGGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCC7490.13152003090457334No Hit
GGCCAGGGCCCCGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTC7480.13134443673781157No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7320.12853493006962308No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7080.12432067006734036No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7010.12309151090000792No Hit
CTCGTAAGGGCCAGGGCCCCGGAGTCCCCAGCCGCTTCTCTGGATCCAAA6960.122213540066199No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6950.12203794589943721No Hit
GACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCCCTACATGGTTTGGCCAAATCAAACAGTAATAGTCAG6920.12151116339915188No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6730.11817487423067806No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6640.11659452672982203No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6630.11641893256306025No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6500.1141362083951571No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6470.11360942589487177No Hit
CCCCTACATGGTTTGGCCAAATCAAACAGTAATAGTCAGCCTCATCTTCA6360.1116778900604922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.11044873089315972No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5960.10465412339002098No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG5940.10430293505649742No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5930.10412734088973565No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5920.10395174672297386No Hit
CTATTACTGTTTGATTTGGCCAAACCATGTAGGGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCC5870.10307377588916497No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5800.1018446167218325No Hit
GTATATGTCGTAGTCACCAACATTGATGTCTCCGGGCAAGGCGCAGGTGA5790.10166902255507072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT200.00757391751.3712812
TCGTATG1150.045.1263440
CTTATAC1450.044.876061
GGTATCA7300.044.0995451
ATGCCGT1200.043.40005544
GTATCAA20050.042.190211
CGTATGC1250.041.5457141
TGGTATC2400.041.382422
GCCGTCT1300.040.1366246
GTGGTAT2500.039.7271231
CCGTTAA350.001395571239.14689616
ATACGTA350.001396776539.14002211
CCGTCTT1250.038.99057447
ATCTCGT1400.036.9370137
CCCACGA1500.036.53710619
ATCTCCG1500.036.5306910
TATCAAC23250.036.2360882
TCTCCGA1550.035.3522811
ATCAACG24500.034.3873063
GCCGTTA400.002688096834.25353615
Produced by FastQC (version 0.11.5)