FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569495
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19700.34592050852070694No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCCC15810.27761437765037444No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13270.2330134592928823No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12460.2187903317851781No Hit
CTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAG12160.21352250678232468No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11680.20509398677775922No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11320.19877259677433518No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10690.187710164268343No Hit
GACTATTACTGTTTGATTTGGCCAAACCATGTAGGGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAG10480.18402268676634562No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10360.18191555676520427No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10360.18191555676520427No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9510.16699005259045296No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9340.16400495175550267No Hit
GTAATAGTCAGCCTCATCTTCAGACTGGAGCCCGGAAATGAGTAAAATCC9310.16347816925521735No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8720.15311811341627232No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8580.15065979508160737No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8530.14978182424779848No Hit
GTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTG8160.14328484007761264No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC8100.14223127507704195No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7930.13924617424209168No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7750.13608547924037964No Hit
GTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAG7690.13503191423980895No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7680.13485632007304715No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7600.13345156673895295No Hit
ATGTAGGGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCC7570.1329247842386676No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7520.1320468134048587No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7370.129412900903432No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7160.1257254234014346No Hit
CTCGTAAGGGCCAGGGCCCCGGAGTCCCCAGCCGCTTCTCTGGATCCAAA7010.12309151090000792No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6960.122213540066199No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6900.12115997506562833No Hit
GGCCAGGGCCCCGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTC6860.1204575983985812No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6820.11975522173153408No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6800.11940403339801052No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6790.11922843923124875No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6530.11466299089544245No Hit
CCCCTACATGGTTTGGCCAAATCAAACAGTAATAGTCAGCCTCATCTTCA6310.11079991922668328No Hit
GACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCCCTACATGGTTTGGCCAAATCAAACAGTAATAGTCAG6170.10834160089201836No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6090.10693684755792414No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6040.10605887672411522No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5990.10518090589030633No Hit
CTATTACTGTTTGATTTGGCCAAACCATGTAGGGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCC5990.10518090589030633No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5980.10500531172354455No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5950.1044785292232592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)