FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265369
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57750.4563886107530689No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39890.3152440118257994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34250.270672033217188No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATT34110.26956563658505933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31210.246647420633823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30830.2436443440609024No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30020.23724304926072948No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAT28570.22578394128511128No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27750.21930361815407207No Hit
GTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26730.21124272840570618No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25720.2032608669882066No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT25230.19938847877575633No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24730.19543706223243973No Hit
GACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGG24660.19488386391637538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24650.19480483558550907No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24210.19132758902739044No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCT24000.18966799407919746No Hit
GAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATC23330.1843730959111532No Hit
GTTCTGACGGTGCTGACAGTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGT22960.1814490476690989No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTA22840.1805007076987029No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21760.17196564796513902No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21270.16809325975268874No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20920.16532726817236712No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20920.16532726817236712No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20850.1647740698563028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20320.16058556832038717No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20070.15860986004872887No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19360.1529988485572193No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18600.14699269541137802No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17810.14074945727293778No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17790.1405914006112051No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGCCAGTT17790.1405914006112051No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17420.1376673523691508No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16890.1334788508332352No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16710.13205634087764123No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16630.13142411423071057No Hit
GTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATG16280.12865812265038895No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15860.12533893275400299No Hit
ACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT15790.12478573443793865No Hit
GCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGA15150.11972792126249338No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15100.11933277960816174No Hit
GTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCC15040.11885860962296373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14580.11522330640311244No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC14120.11158800318326118No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC14120.11158800318326118No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14010.11071869154373151No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13650.10787367163254355No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13480.1065301900078159No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13320.10526573671395457No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13070.10329002844229629No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC12980.10257877346449928No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12950.1023416884719003No Hit
GGACTACAGTAACAAGCGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCC12890.10186751848670231No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12840.10147237683237065No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12710.10044500853110831No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12660.10004986687677665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.044.7578621
GGTATCA8100.043.897641
ATCAACG28100.038.8486443
TATCAAC29050.037.978892
CAACGCA28700.037.771115
AACGCAG28850.037.336166
ACGCAGA30100.035.7856527
CGCAGAG30600.035.425858
AGAGTAC31250.034.35861611
GTAGTTT1904.4201443E-1034.0265870
GCAGAGT32500.033.248919
CAGAGTA34500.031.22168710
TCAACGC34850.031.2068374
AGTACGG29800.031.18017813
GAGTACG29950.030.90911512
TTAACGG1103.631976E-828.25265735
TACTCGC500.007237431627.978758
TAGTCAC500.007741661727.5952832
TGCAACG500.00783034427.5309438
TTTAACG1155.6041245E-827.01785534
Produced by FastQC (version 0.11.5)