FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232379
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.039.8827971
GTATCAA33650.038.1436231
CTTATCG1555.131369E-934.5361970
TATCAAC38200.033.418822
TCAACGC38450.033.2015344
CAACGCA39500.032.406075
ATCAACG39600.032.150453
AACGCAG40150.032.052856
ACGCAGA42350.030.3840647
CGCAGAG44250.029.0794378
GTGGTAT5450.028.4128111
CAGAGTA45800.028.2455510
AGAGTAC45750.028.12601511
GCAGAGT48500.026.6731199
TGGTATC5450.026.5175482
AGTACGG38050.024.32444613
GAGTACG39250.023.49215312
TACGGGT4700.021.9618415
GTACGGG42050.021.92876214
TTTAACG3050.021.48610334
Produced by FastQC (version 0.11.5)