FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232379
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14090.1143317112673942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.043.7566761
GTATCAA35850.039.1233331
TATCAAC41000.033.659552
TCAACGC40900.033.435064
CAACGCA41300.033.1930855
ATCAACG41250.033.1688653
AACGCAG42900.032.03526
ACGCAGA44850.030.6423637
GTGGTAT5150.030.1117441
CGCAGAG45950.029.9088158
AGAGTAC47100.029.39739611
CAGAGTA47850.028.93662510
GCAGAGT51100.027.0289949
TGGTATC5600.025.8112492
AGTACGG40250.025.77898413
GAGTACG41650.024.91246212
TACGGGG21450.023.22646115
GTACGGG45400.022.70336214
GTTAGAC953.1405393E-421.7119643
GTCTTAC1605.7876605E-821.5382611
Produced by FastQC (version 0.11.5)