FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624403
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45370.7266140617517852No Hit
ACATTACACTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT39860.638369770805073No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34880.5586135876989701No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCT31640.5067240227865657No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29440.47149036759913066No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28680.4593187412616532No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28010.44858849172729787No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27780.4449049732304297No Hit
GTGTAATGTTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC26910.4309716641335804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26560.4253663098992157No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26080.41767896694922996No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGAAGTTCTTATCGTTGGAGTTGTATAAAATATT24170.3870897481274113No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC22460.3597035888680868No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC21710.3476921155087339No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21250.34032507851499755No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21010.3364814070400046No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20850.3339189593900093No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCA20460.32767299324314586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20430.3271925343087717No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19800.3171028966869154No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19500.3122983073431742No Hit
ATTTTATACAACTCCAACGATAAGAACTTCTTAGCTTGGTATCAGCAGAA19400.31069677756192715No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAACATTACACTTCT19180.30717341204318366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18960.3036500465244401No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18690.2993259161150731No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCC18080.28955658444946614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17240.2761037342869909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16720.26777577942450626No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATA16670.2669750145338828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16330.2615298132776428No Hit
GGCTGAAGATGTGGCAATTTATTACTGTCAGCAACATTACACTTCTCCTC16010.25640491797765225No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15750.2522409405464099No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAACATTACACTTCTCCTCCGACGTT15510.248397269071417No Hit
GTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15440.24727619822454408No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15440.24727619822454408No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14780.2367061016683136No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14310.22917891169645246No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14210.2275773819152054No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT14000.2242141693745866No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13750.22021034492146896No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13570.2173275913152243No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13080.2094800953871138No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13030.20867933049649023No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATACAACTCCAA12780.20467550604337262No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12640.20243336434962678No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12330.19746862202776094No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATACAACTCCAACGATAAGAACTT12220.19570693926838917No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12160.19474602139964095No Hit
GCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGG12140.19442571544339154No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11900.19058204396839862No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11850.1897812790777751No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11770.18850005525277747No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11760.18833990227465275No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC11640.18641806653715629No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11600.18577745462465747No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11450.1833751599527869No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11280.18065255932466692No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11140.17841041763092105No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11120.17809011167467165No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11000.1761682759371752No Hit
GGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTTCATC10840.1736058282871799No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10800.1729652163746811No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10760.17232460446218228No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAAGAAGTTCTTATCGTTGGAGTTG10650.17056292170281054No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGATCAGGGACC10560.16912154489968817No Hit
ATTTATTACTGTCAGCAACATTACACTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA10530.16864108596531407No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10450.16735986214031642No Hit
GCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATT10440.16719970916219173No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10220.1636763436434482No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10190.16319588470907412No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10180.1630357317309494No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10150.1625552727965753No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10090.16159435492782706No Hit
CTTCTTAGCTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATC10080.16143420194970234No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10060.16111389599345294No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10030.16063343705907884No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG9930.15903190727783179No Hit
GATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9880.15823114238720826No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9860.15791083643095885No Hit
CTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGG9580.1534265530434671No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9530.1526257881528436No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9430.15102425837159655No Hit
ATCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGATA9390.15038364645909774No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9340.1495828815684742No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9320.1492625756122248No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9290.14878211667785068No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8980.14381737435598482No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8980.14381737435598482No Hit
GCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTC8860.14189553861848836No Hit
CCACTGAATCGATCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAAT8840.14157523266223895No Hit
GCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTT8660.1386924790559943No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8570.13725110225287193No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8540.13677064331849784No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8300.1329269718435049No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGAGAAGTGTAATGTTGCTGACAGTAATAAATTGCCACATCTTCAGC8270.13244651290913081No Hit
GCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTC8250.13212620695288138No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGT8190.13116528908413316No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8120.13004421823726023No Hit
TTATTACTGTCAGCAACATTACACTTCTCCTCCGACGTTCGGCCAAGGGA8090.1295637593028861No Hit
TTACACTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTT7890.12636069974039205No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7860.1258802408060179No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC7860.1258802408060179No Hit
CATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA7820.12523962889351908No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7800.12491932293726968No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC7640.12235687528727442No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC7600.12171626337477558No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7590.12155611039665087No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTA7590.12155611039665087No Hit
ACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC7580.12139595741852617No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7560.12107565146227676No Hit
CTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACT7550.12091549848415206No Hit
CCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAA7320.11723197998728385No Hit
ATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGAGAAGTGTAATGTTG7200.1153101442497874No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG7040.11274769659979213No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7040.11274769659979213No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7010.11226723766541799No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG7010.11226723766541799No Hit
GTATCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCAT6980.1117867787310439No Hit
GGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCT6900.11050555490604627No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6870.11002509597167213No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6850.10970479001542273No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA6840.10954463703729803No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6810.10906417810292392No Hit
GTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTA6760.10826341321230037No Hit
GTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA6740.10794310725605097No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGC6740.10794310725605097No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT6560.1050603536498063No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG6490.10393928280293335No Hit
ACTATAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGGAGACACGTCC6400.10249790599981103No Hit
GGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTA6390.10233775302168631No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG6360.1018572940873122No Hit
GTATAAAATATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA6360.1018572940873122No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC6350.1016971411091875No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG6340.1015369881310628No Hit
CTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCA6290.10073622324043928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)