FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624403
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44510.7128409056330607No Hit
ACATTACACTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT38940.6236356968176001No Hit
GTGTAATGTTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31270.5007983625959517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29400.47084975568663184No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCT28540.45707659956790725No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27550.44122145473356145No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27510.44058084282106263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26090.4178391199273547No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25750.41239391867111463No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25710.4117533067586159No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGAAGTTCTTATCGTTGGAGTTGTATAAAATATT22270.35666068228371745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21010.3364814070400046No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC20820.3334385004556352No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20680.33119635876188935No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC20520.3286339111118941No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20480.32799329919939524No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19700.31550136690566827No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19270.308614788846306No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18860.3020485167431931No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18770.30060713994007077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18630.2983649982463249No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCA18420.29500178570570607No Hit
ATTTTATACAACTCCAACGATAAGAACTTCTTAGCTTGGTATCAGCAGAA17950.28747459573384493No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAACATTACACTTCT17500.2802677117182332No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16820.26937730920575337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16510.2644125668838875No Hit
GGCTGAAGATGTGGCAATTTATTACTGTCAGCAACATTACACTTCTCCTC16300.2610493543432687No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCC16270.2605688954088946No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15850.253842470327657No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATA15780.2527213994807841No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAACATTACACTTCTCCTCCGACGTT15540.24887772800579114No Hit
GTCCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCGGATGCCCGGGT15410.24679573929016996No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15400.24663558631204527No Hit
GTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15390.24647543333392055No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15340.24567466844329705No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14530.23270227721519599No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14480.23190151232457243No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14370.23013982956520068No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT14270.22853829978395362No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGATCAGGGACC14150.2266164640464572No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13900.22261263959333955No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13280.21268315494960785No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12740.2040348941308738No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGATCAGGGACCCCG12420.19890999883088326No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11930.1910625029027727No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11860.1899414320558998No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATACAACTCCAA11720.1876992903621539No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11690.1872188314277798No Hit
GCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGG11670.1868985254715304No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11530.18465638377778454No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11510.18433607782153513No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11500.18417592484341044No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATACAACTCCAACGATAAGAACTT11300.18097286528091633No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11130.17825026465279636No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10900.17456674615592815No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAAGAAGTTCTTATCGTTGGAGTTG10900.17456674615592815No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10730.17184414552780816No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC10640.17040276872468582No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10600.16976215681218698No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10540.16880123894343876No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10520.16848093298718936No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10370.1660786383153188No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10350.16575833235906937No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10330.16543802640281996No Hit
ATTTATTACTGTCAGCAACATTACACTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA10210.16351619066532352No Hit
GGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTTCATC9980.15983267216845531No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9910.15871160132158238No Hit
CTTCTTAGCTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATC9860.15791083643095885No Hit
GATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9840.15759053047470944No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9830.15743037749658473No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9600.15374685899971652No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9570.15326640006534242No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9540.1527859411309683No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9440.15118441134972124No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9390.15038364645909774No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9360.14990318752472362No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9200.14734073987472834No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9060.14509859818098247No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGAGAAGTGTAATGTTGCTGACAGTAATAAATTGCCACATCTTCAGC9060.14509859818098247No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9020.14445798626848366No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8990.14397752733410954No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8920.1428564564872366No Hit
GCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTC8910.14269630350911192No Hit
GTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA8870.14205569159661308No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8860.14189553861848836No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8820.14125492670598955No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8800.14093462074974014No Hit
GCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATT8720.13965339692474252No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG8590.13757140820912134No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8560.13709094927474724No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8560.13709094927474724No Hit
ATCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGATA8520.13645033736224843No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8510.1362901843841237No Hit
GCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTT8490.1359698784278743No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGAT8480.1358097254497496No Hit
TTACACTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTT8350.13372773673412844No Hit
CTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGG8250.13212620695288138No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8190.13116528908413316No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8140.13036452419350966No Hit
CAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA8080.12940360632476142No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGT7980.12780207654351436No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7870.1260403937841426No Hit
CTTCTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACT7830.1253997818716438No Hit
TTATTACTGTCAGCAACATTACACTTCTCCTCCGACGTTCGGCCAAGGGA7820.12523962889351908No Hit
CCACTGAATCGATCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAAT7560.12107565146227676No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7460.11947412168102972No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7400.1185132038122815No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7340.11755228594353326No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7270.11643121509666034No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7220.11563045020603681No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7200.1153101442497874No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA7160.11466953233728859No Hit
GTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTA7140.11434922638103918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7080.11338830851229094No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC6980.1117867787310439No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTA6950.11130631979666977No Hit
CATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA6910.11066570788417096No Hit
CCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAA6900.11050555490604627No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGC6860.10986494299354743No Hit
ATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGAGAAGTGTAATGTTG6780.10858371916854981No Hit
GGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC6770.1084235661904251No Hit
GCATCTACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTC6770.1084235661904251No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT6760.10826341321230037No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT6650.10650173045292864No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA6620.10602127151855452No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG6600.10570096556230511No Hit
ACCCGGGCATCCGGGGTCCCTGATCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC6570.105220506627931No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC6550.1049002006716816No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6520.10441974173730749No Hit
GTATCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCAT6450.10329867089043454No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6380.1021776000435616No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC6320.10121668217481339No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG6300.10089637621856398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)