FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207715
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62450.5170921947644933No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44100.3651523745254468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39940.3307071618717992No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37160.3076884861080636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36820.3048732523815635No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35900.2972555611216222No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35420.29328111350773983No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34410.28491821332019557No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG32600.26993123377618067No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA31970.26471477128296No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCAC31700.2624791445001511No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31570.2614027316047246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30090.24914818479525383No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29820.24691255801244497No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC28770.2382184538570772No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27350.22646071299934173No Hit
GCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT27340.22637791200738586No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA26640.220581842570474No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26400.21859461876353276No Hit
GACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25840.2139577632140033No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25760.21329535527835625No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25240.20898970369665032No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24860.20584326600232672No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24340.20153761442062074No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24300.20120641045279722No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23650.19582434597566478No Hit
GATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA23400.19375432117676772No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22790.1887034606674588No Hit
ACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA22490.1862194309087823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22050.1825761872627234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21930.18158257535925282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21790.18042336147187044No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20940.17338527715562033No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA20590.1704872424371644No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20330.1683344166463114No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC19970.1653535809358996No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA19650.16270394919331135No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT19460.16113073034614955No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19370.1603855214185466No Hit
GTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGA19130.1583982976116054No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19090.15806709364378185No Hit
GATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA18900.15649387479662005No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18850.15607986983684063No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18630.1542582480138112No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA18200.1506978053597082No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17560.14539854187453166No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA17550.1453157408825758No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17260.14291451211585515No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16770.13885726351001684No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16670.13802925359045803No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16460.13629043275938446No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15990.13239878613745792No Hit
TTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTC15910.13173637820181083No Hit
CTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTT15800.13082556729029612No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15400.12751352761206078No Hit
GTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAATCT15350.1270995226522814No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14980.12403588594991367No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14950.12378748297404603No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14780.12237986611079602No Hit
GCAGAAACCAGGGAAAGCCCCTAATCTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTT14780.12237986611079602No Hit
CTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA14580.12072384627167833No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14540.1203926423038548No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14420.11939903040038419No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14280.11823981651300182No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14090.11666659766584003No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14050.11633539369801651No Hit
CCATATTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT13910.11517617981063413No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13880.11492777683476647No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13860.11476217485085471No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGCCATATTGTAAGTCTGTTGACA13860.11476217485085471No Hit
ATTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT13740.11376856294738412No Hit
GTGCTAATGCTGTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA13690.11335455798760469No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13640.11294055302782527No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13590.11252654806804586No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13520.11194694112435467No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13460.11145013517261937No Hit
ATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGAC13460.11145013517261937No Hit
GAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG13370.11070492624501642No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13320.110290921285237No Hit
GATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTA13250.10971131434154581No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13170.10904890640589873No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13050.10805529450242812No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13030.10788969251851636No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGCCATATTGTAAGTCTGTTGACAG13010.1077240905346046No Hit
GGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC12920.10697888160700166No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12890.10673047863113401No Hit
ACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA12850.10639927466331046No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12730.10540566275983987No Hit
CCCCTAATCTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCG12650.1047432548241928No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12570.10408084688854571No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12460.103170035977031No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12280.1016796181218251No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12220.1011828121700898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCTA200.007143466752.14071747
GTATCAA26050.041.081521
GTGGTAT3700.040.9208641
TATCAAC28700.037.2882772
ATCAACG29900.035.791763
TCAACGC30150.035.4949844
CAACGCA30450.035.371295
AACGCAG31400.034.301146
TCGTATG1350.033.32113640
CGTATGC1400.032.15251541
AGAGTAC34000.031.47178611
ACGCAGA34400.031.3058727
CGCAGAG34300.031.2968358
TGTTAGA11700.030.61423370
CGGGACG450.00467984830.58984817
GCAGAGT35700.030.1658829
GTCTAGA802.8181403E-630.1093861
CTCGTAT1501.8189894E-1229.97903839
TATACAC2500.028.9050123
CGCATAC500.007731134527.60287335
Produced by FastQC (version 0.11.5)