FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207715
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58660.48571061881321337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36810.30479045138960764No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36370.30114720774354875No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36250.30015359584007817No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35610.2948543323549016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34810.2882302529984309No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33940.2810265666982691No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31550.26123712962081286No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30280.25072140364241563No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA29900.24757496594809206No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCAC29500.24426292626985674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27310.22612950903151818No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27090.22430788720848877No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC26880.22256906637741522No Hit
GCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT26750.2214926534819887No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA26590.22016783761069458No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25400.21031451956794442No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24680.20435284814712082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24650.20410444517125317No Hit
GACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24390.20195161938040018No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24390.20195161938040018No Hit
ACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24120.19971599259759132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24040.19905358466194423No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24040.19905358466194423No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24000.1987223806941207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23920.19805997275847365No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23830.19731476383087068No Hit
GATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA23090.1911874904261353No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT22760.18845505769159115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22080.18282459023859107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21060.1743788890590909No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21000.17388208310735562No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA20300.16808601367044376No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20230.1675064067267526No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC19970.1653535809358996No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19860.1644427700243849No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19720.16328355613700252No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19050.1577358896759583No Hit
GATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA18690.1547550539655465No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA18630.1542582480138112No Hit
GTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGA18620.15417544702185532No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA18160.15036660139188468No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17820.14755136766538463No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17790.14730296468951698No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17380.14390812401932573No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17080.14142409426064925No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16920.1400992783893551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16760.13877446251806097No Hit
CTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTT16570.13720124367089917No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA16170.13388920399266382No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16160.13380640300070795No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16100.13330959704897263No Hit
TTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTC15780.13065996530638438No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15470.12809313455575197No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15050.12461549289360487No Hit
GTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAATCT15010.12428428892578133No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14690.12163465718319307No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14410.11931622940842831No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14100.11674939865779592No Hit
CTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA13920.11525898080259002No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13900.11509337881867826No Hit
ATTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT13860.11476217485085471No Hit
CCATATTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAAT13750.11385136393933998No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13720.11360296096347233No Hit
CTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13600.11260934906000174No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13560.1122781450921782No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGCCATATTGTAAGTCTGTTGACA13430.11120173219675172No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13370.11070492624501642No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13290.11004251830936936No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGCCATATTGTAAGTCTGTTGACAG13240.10962851334958992No Hit
GGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC13110.10855210045416343No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12970.10739288656678107No Hit
GTGCTAATGCTGTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA12960.10731008557482517No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12920.10697888160700166No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12870.10656487664722222No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12840.10631647367135458No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12770.1057368667276634No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12640.10466045383223692No Hit
ACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA12610.10441205085636926No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12570.10408084688854571No Hit
ATCTGGGACAGATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAACAGA12460.103170035977031No Hit
CCCCTAATCTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCG12410.10275603101725157No Hit
GAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG12380.10250762804138393No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12330.10209362308160452No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12330.10209362308160452No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12240.10134841415400156No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12220.1011828121700898No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12190.10093440919422215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25850.041.289281
TATCAAC28150.037.498982
GTGGTAT3400.036.4552231
ATCAACG29900.035.1484223
TCAACGC29900.034.910024
CAACGCA29900.034.790865
AACGCAG31000.033.776446
CGCAGAG34250.030.5713778
ACGCAGA34200.030.5156927
AGAGTAC34400.030.43807211
GCAGAGT36100.029.00479
CAGAGTA38950.026.88240410
AGTACGG28950.026.56288113
GAGTACG29650.025.81997912
ACGGGGC2800.025.75187916
CCTATAC1101.1991833E-624.9777243
TTATACT1301.9346407E-723.7709644
CAGTTCG24500.023.64494770
CAGCGCT2500.023.348374
TACGGGG17350.022.75862715
Produced by FastQC (version 0.11.5)