FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774947
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCCGGAGTGGGTTG28020.36157311403231446No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26610.34337832135617014No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21960.2833742178497368No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT21080.2720186025624978No Hit
GAGTATTAGTAGCTGGTTGGCCTGGCATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT20240.26117915160649696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18810.24272627676473357No Hit
GTAATACACGGCCGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAAATACA18190.23472572963054247No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC15880.20491723950154012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15840.20440107517030198No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15620.20156217134849222No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15470.19962655510634922No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT15290.1973038156157776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15240.19665861020172992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15100.19485203504239643No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15090.19472299395958692No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14980.19330354204868203No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14830.19136792580653902No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14570.18801285765349113No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13730.1771734066974903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13620.1757539547865854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13150.1696890238945373No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGCATCAGCAGAAA13020.16801148981801337No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12840.16568875032744174No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12780.16491450383058454No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA12530.1616884767603462No Hit
CTATAATAATTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12450.16065614809786993No Hit
TTATTATAGTGTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTC12350.15936573726977457No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT11990.15472025828863137No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11810.15239751879805974No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11760.15175231338401207No Hit
GTGTATTACTGTGCTATAGGCCTCTACGGTGGCTGGAACTACTATTACGA11660.1504619025559167No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11540.1489134095622023No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11420.1473649165684879No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11380.14684875223724977No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11250.14517121816072584No Hit
GTCCATGTAGTGGTCGCTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC11080.14297751975296374No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC10980.1416871089248684No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCGACCACTACATGGACTGGG10980.1416871089248684No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA10840.13988053376553494No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG10600.1367835477781061No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10570.1363964245296775No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10540.1360093012812489No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10390.13407368503910588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10310.13304135637662964No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAATTTATTACTGCCAACACTATAATAATTAT10190.13149286338291521No Hit
GTATTAGTAGCTGGTTGGCCTGGCATCAGCAGAAACCAGGGAAAGCCCCT9890.1276216308986292No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9630.1242665627455813No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9440.12181478217220015No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9240.11923396051600947No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9160.11820163185353322No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA9050.11678217994262832No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8910.11497560478329485No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8870.11445944045205673No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8770.11316902962396139No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGTTTTCATTTGGTGAT8730.11265286529272324No Hit
CAGTAATACACGGCCGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAAATA8680.11200765987867556No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8520.10994300255372302No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC8490.10955587930529442No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8480.1094268382224849No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTGCTAAACTAGA8460.10916875605686581No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC8360.10787834522877049No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8310.10723313981472282No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8280.10684601656629422No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8220.10607177006943701No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8140.10503944140696074No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA8080.10426519491010353No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG8050.10387807166167493No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG7990.10310382516481773No Hit
TCTATAAGGCGTCTAGTTTAGCAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT7960.10271670191638912No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7950.10258766083357958No Hit
GTATTACTGTGCTATAGGCCTCTACGGTGGCTGGAACTACTATTACGATA7950.10258766083357958No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG7940.10245861975077006No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7890.10181341433672239No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATGCCAGGCCAACCAGCTACTAATACTCTGA7830.10103916783986518No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7800.10065204459143656No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT7800.10065204459143656No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGC7790.10052300350862704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4550.048.5286061
GTATCAA12500.043.8850251
TCGTATG1050.042.8702340
CGTATGC1100.040.93483741
CTTATAC1100.040.7736781
ACGAGAC1150.038.9958822
TATCAAC14250.038.490672
TATACAC1350.038.329283
ATCAACG14950.036.919173
TAACAGT9650.036.65570470
CAACGCA15200.036.53895
TCAACGC15300.036.074624
AACGCAG15400.036.064376
CGCAGAG16400.034.071278
ACGCAGA16450.033.758037
CTCGTAT1253.6379788E-1233.2344639
GACCCTA1350.033.2294326
GCCGTCT1154.3655746E-1133.19358446
AGAGTAC16700.033.0461311
CTTGAAA1600.032.7204157
Produced by FastQC (version 0.11.5)