FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075141
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11620.10807884733258244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38100.041.1928631
GACGCAT350.001388910739.18816825
GGTATCA15450.037.787771
TATCAAC42700.036.3910942
TCAACGC44400.035.0276874
ATCAACG44750.034.6900863
CAACGCA44700.034.63455
AACGCAG46000.033.8046156
ACGCAGA48000.032.324737
CGCAGAG48350.032.0907368
AGAGTAC48350.032.01989711
GTGGTAT5550.031.557891
CAGAGTA49700.031.1501410
GCAGAGT50900.030.6849239
TGGTATC5850.029.318312
GAGTACG37550.027.63825812
AGTACGG37450.027.62059813
GTACGGG40050.025.6564614
TACGGGG15400.025.3549115
AGTACTT21450.024.4310513
Produced by FastQC (version 0.11.5)