FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018838
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46420.45561708534624745No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40570.3981987322812851No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39840.3910337070270249No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC39700.389659592594701No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33320.32703923489308406No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33080.32468361015195746No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC32770.32164092819466883No Hit
ATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC30870.3029922323274161No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGG30720.30151996686421195No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30720.30151996686421195No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT28380.2785526256382271No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27950.27433213131037515No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGC27720.2720746576001288No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC26490.26000208080185466No Hit
GAAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG25680.2520518473005522No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25430.24959807152854524No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC25360.24891101431238333No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25200.24734059781829887No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24860.24400346276836948No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24250.23801624988467254No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTC24000.2355624741126656No Hit
GTCTGAGGATGAGGCTGATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGAC23820.23379575555682064No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23430.2299678653524898No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTA22870.22447140762319429No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20740.20356523804569518No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT20730.2034670870148149No Hit
GATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTA20640.20258372773689243No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20300.199246592686963No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20160.19787247825463913No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19760.19394643701942801No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19120.18766477104309026No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19030.18678141176516777No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA18870.18521099527108334No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18850.18501469320932276No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCA18740.18393503186963972No Hit
CCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA17670.17343287156545006No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17370.17048834063904172No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17160.1684271689905559No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGG16520.16214550301421818No Hit
GTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAG16340.16037878445837317No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16020.1572379514702043No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15870.15576568600700014No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15720.15429342054379597No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15600.15311560817323266No Hit
GTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTT15530.1524285509570707No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15470.15183964477178902No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15470.15183964477178902No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTTCTGTGCAG15280.14997477518506375No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15010.14732469735129627No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14960.14683394219689488No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCA14850.14575428085721184No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14440.14173008859112046No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGG14370.14104303137495855No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14160.13898185972647273No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13890.13633178189270523No Hit
GACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCT13600.13348540199717718No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13600.13348540199717718No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13530.13279834478101524No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGT13360.13112977725605052No Hit
GGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC13200.12955936076196609No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13070.12828339736052247No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTC12980.12740003808259998No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG12970.12730188705171971No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12850.12612407468115638No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG12620.12386660097090998No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12560.12327769478562833No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG12430.12200173138418474No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12420.12190358035330447No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC12250.12023501282833973No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12250.12023501282833973No Hit
GGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG12050.11827199221073419No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC12040.11817384117985391No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11930.11709417984017087No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11770.11552376334608642No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTA11650.1143459509755231No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC11520.1130699875740795No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11420.11208847726527672No Hit
GATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAG11400.11189217520351617No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11300.11091066489471339No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11220.11012545664767118No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11170.10963470149326977No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAACCTGGTACCAGCAGCTCCCAGGGGCGGCCCC11080.10875134221534728No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11060.10855504015358673No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10880.10678832159774174No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10830.10629756644334036No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC10760.1056105092271784No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10600.10404009273309398No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG10380.10188077005372788No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA10330.10139001489932649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT200.00740792251.6629814
TCGTATG800.047.5678540
CTACGCT950.043.5291728
GGTATCA6350.042.3108441
GTATCAA15650.041.598561
GTGGTAT2250.039.803541
CTCGTAT952.1464075E-1036.40480839
TGGTATC2300.035.941232
TATCAAC18450.035.4704552
CGTATGC905.0349627E-934.60674341
ACTACGC1201.8189894E-1234.4572127
ATCAACG19450.033.4696923
CAACGCA19700.033.0449525
TCAACGC19750.032.9612924
AACGCAG20650.031.5247236
TCTCGTA1101.036824E-931.42348338
AGAGTAC21800.029.70080811
CAGAGTA21950.029.65474310
CGCAGAG22000.029.5873458
ACGGGTA605.4722803E-428.70447516
Produced by FastQC (version 0.11.5)