FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874487
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACGACGGGCCCCTGGACAAGGGCTTGAGTGGATGGGTTGGATCAACCCTAACAGTGGTGCCACAAACTATG23550.2693007443220997No Hit
GTATACATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTA16780.19188392737685067No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16630.19016863601174172No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA16480.18845334464663283No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16450.18811028637361105No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15360.17564583578715293No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAG15160.17335878063367435No Hit
CCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGGGATCCCT14750.16867031756904335No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14710.16821290653834764No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14170.16203785762395553No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13630.15586280870956343No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG13590.1554053976788677No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12650.14465623845751852No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12590.14397012191147496No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12420.1420261250310182No Hit
CCATGTATACTGTGCTGATGGACGTGTCCCTGGTCATGAGGACCCAGCCCTGAAACTTCTGTGCATAGTTTGTGG12360.1413400084849746No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11790.13482190129756075No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11650.13322096269012573No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11590.13253484614408217No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11570.13230614062873433No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11190.12796073583712508No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11190.12796073583712508No Hit
CCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAGCCTCATCCATA11170.1277320303217772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10880.12441580034923332No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10640.12167133416505906No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAG10590.12109957037668942No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10460.11961298452692835No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10290.1176689876464716No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10280.11755463488879767No Hit
GACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG9700.11092217494370986No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9660.11046476391301414No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT9650.11035041115534021No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9640.1102360583976663No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC9310.10646241739442666No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9260.10589065360605704No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9150.10463277327164384No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG9060.1036035984525785No Hit
CCCTAACAGTGGTGCCACAAACTATGCACAGAAGTTTCAGGGCTGGGTCCTCATGACCAGGGACACGTCCATCAG9050.10348924569490456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.035.844061
TCAACGC21650.035.710184
ATCAACG21850.035.3833123
CAACGCA21850.035.225355
ATCGTAT400.002553796234.61548639
AACGCAG22600.034.056376
GTATCAA24650.033.186251
CGCAGAG24150.031.865098
GACAGGT2000.031.064851
AGAGTAC25000.030.9197111
CAGAGTA25150.030.87250910
GGACGTA450.004615166730.6760723
ACCGTCG450.00461645830.6743138
TATCAAC26550.030.5495782
ACGCAGA25550.030.1207797
GTATTAG1501.8189894E-1229.91431
GCAGAGT26150.029.5599549
TACTATG7450.029.08034370
CTTATAC1353.0377123E-1028.1245541
TCGTATG1004.851481E-727.70510340
Produced by FastQC (version 0.11.5)