FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373887
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13160.3519780040493518No Hit
GACTTACAGCGTCCCTCTAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10950.2928692358921278No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10030.26826287086740114No Hit
CTGTAAGTCTGTTGACAATAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC9600.25676206982323535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8480.22680649501052455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8000.2139683915193628No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7500.20059536704940262No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7460.1995255250918058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7380.19738584117661218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7150.1912342499204305No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAACAGACTTACAGCGTC7060.18882710551583767No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGACCGCTAGCAG6720.17973344887626475No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6600.1765239230034743No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGACCGCTAGCAGCTACTTAAATTGGTATCAGCAGAAA6490.17358185762008307No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT6440.17224455517308707No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6210.1660929639169054No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6200.16582550342750618No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6100.16315089853351414No Hit
CTCCTGATCTATGGTGCATTCACTTTACAAAGTGGAGTCCCATCAAGGTT5850.15646438629853404No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5740.1535223209151428No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5600.14977787406355397No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC5500.14710326916956193No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5490.14683580868016272No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5470.14630088770136432No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCGTAGAGGTCAACCTCAGGGACA5110.136672310082993No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTAGAGGGACG5040.13480008665719856No Hit
GATCTATGGTGCATTCACTTTACAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA4870.1302532583374121No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4670.12490404854942803No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4600.12303182512363361No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA4550.12169452267663759No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC4540.12142706218723838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4510.12062468071904078No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4510.12062468071904078No Hit
GTTTATGGTGGGTCCTTCAGTGATTACTCCTGGGGCTGGATCCGCCAGTC4500.12035722022964157No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT4360.11661277337805273No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT4320.11554293142045592No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4290.1147405499522583No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGA4290.1147405499522583No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4220.11286832652646388No Hit
GATCAGGAGAGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATTTAAGT4150.11099610310066946No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4150.11099610310066946No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4020.10751911673847982No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCACTTTGTAAAGT3920.10484451184448777No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAACAGA3740.10003022303530212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)