FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373887
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12920.3455589523037709No Hit
GACTTACAGCGTCCCTCTAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10250.2741470016341836No Hit
CTGTAAGTCTGTTGACAATAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC9000.24071444045928314No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8900.23803983556529112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7660.20487473487978985No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAACAGACTTACAGCGTC7410.1981882226448098No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7190.19230409187802733No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7000.18722234257944245No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6730.18000090936566396No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6500.1738493181094823No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGACCGCTAGCAGCTACTTAAATTGGTATCAGCAGAAA6460.17277947615188546No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6410.17144217370488943No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGACCGCTAGCAG6360.17010487125789345No Hit
CTCCTGATCTATGGTGCATTCACTTTACAAAGTGGAGTCCCATCAAGGTT6300.1685001083214982No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6260.16743026636390138No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT6210.1660929639169054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6140.16422074049111096No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6040.16154613559711892No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC5830.15592946531973564No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5520.14763819014836033No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5120.13693977057239218No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA5050.13506754714659777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5010.13399770518900095No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTAGAGGGACG5000.13373024469960176No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC4900.13105563980560972No Hit
GATCTATGGTGCATTCACTTTACAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA4800.12838103491161768No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCGTAGAGGTCAACCTCAGGGACA4650.12436912757062962No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT4570.122229443655436No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4560.12196198316603679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4540.12142706218723838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4510.12062468071904078No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4510.12062468071904078No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4510.12062468071904078No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4490.12008975974024239No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT4490.12008975974024239No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4440.11875245729324635No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4430.11848499680384716No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT4390.11741515484625033No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC4360.11661277337805273No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCACTTTGTAAAGT4350.11634531288865352No Hit
GTTTATGGTGGGTCCTTCAGTGATTACTCCTGGGGCTGGATCCGCCAGTC4240.11340324750526228No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGA4040.10805403771727823No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT3740.10003022303530212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACATA250.00906011549.08933669
GTATCAA9400.046.0509871
TCCGAAC350.001281361739.8290753
GTGTATA350.001321911739.577531
TATCAAC11300.038.2308432
GTGGTAT1051.6370905E-1136.2794041
ATCAACG11850.036.1018263
CAACGCA12100.035.607685
TCAACGC12050.035.4693874
AACGCAG12200.035.3158156
GTATTAT400.002546558934.6303441
TAGTGAT1855.329639E-1033.4070970
AGAGTAC12700.033.3826211
CTAGTGA1503.6379788E-1232.72622369
ACGCAGA13350.032.273637
CGCAGAG13350.032.273638
CAGAGTA13650.031.56431810
TAGTTCG450.004340868431.05796657
GCAGAGT13900.030.9966139
GTCTAGT1451.4551915E-1129.47956867
Produced by FastQC (version 0.11.5)