FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703080
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24860.35358707401718154No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17400.2474825055470217No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17340.24662911759685954No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17140.24378449109631906No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT16150.2297035899186437No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15280.2173294646412926No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14440.2053820333390226No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAGTGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG14320.2036752574386983No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTTCTTAGCCTGGTACCAACAGAAAGTTGGCCAGGCTCCCAGGCT13150.18703419241053648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12860.1829094839847528No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12720.18091824543437446No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12540.17835808158388802No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGTT12420.17665130568356374No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12180.17323775388291518No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12130.17252659725778005No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGGCCTTGGACGTTCGGCCAAGGGA11880.16897081413210446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11810.16797519485691528No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11410.16228594185583434No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG11180.1590146213802128No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10960.15588553222961823No Hit
GTCTTTGTCTCCAGGAGAAGTGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTTCTTAGCCTGGTA10710.15232974910394267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10280.14621380212778062No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10230.1455026455026455No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10120.14393810092734824No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9880.14052454912669965No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9460.13455083347556465No Hit
GAGTGTTAACAGCTTCTTAGCCTGGTACCAACAGAAAGTTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC9090.12928827444956478No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9020.1282926551743756No Hit
GTGTTAACAGCTTCTTAGCCTGGTACCAACAGAAAGTTGGCCAGGCTCCC8910.12672811059907835No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8630.12274563349832168No Hit
TCTATGATGCATCCAACAGGGCCACTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8590.12217670819821358No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8460.12032770097286226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8140.11577629857199749No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCA8110.11534960459691641No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8040.11435398532172725No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7930.11278944074642999No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7300.1038288672697275No Hit
GGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7270.10340217329464642No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7140.1015531660692951No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7130.10141093474426807No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7090.10084200944415998No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAGTGGC7080.10069977811913297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5750.041.947881
GTATCAA14450.040.7763251
TATCAAC16550.034.9776882
ATCAACG17000.034.0518073
TCAACGC17000.034.0518074
CAACGCA17600.032.890955
AACGCAG17600.032.890956
GGACGCA553.2781763E-431.32694211
CGCAGAG18700.031.1404518
ACGCAGA19350.030.094397
AGAGTAC19500.030.04173511
CGTTTAG604.8924866E-429.25739963
GCAGAGT20400.028.7143219
CAGAGTA21200.027.47018410
AGTACGG15500.027.12513
GAGTACG15850.026.74154912
GGAACGA658.669751E-426.53005626
TACTGAC658.716711E-426.5055272
GTATAAG1850.026.075711
TAACCGT700.001343013724.6122767
Produced by FastQC (version 0.11.5)