FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703080
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23590.3355236957387495No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15920.22643226944302214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15910.22629003811799508No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT15560.22131194174204927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15440.21960516584172496No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14940.2124935995903738No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14490.2060931899641577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13960.19855492973772543No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAGTGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG12780.1817716333845366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12620.17949593218410423No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12280.1746600671331854No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTTCTTAGCCTGGTACCAACAGAAAGTTGGCCAGGCTCCCAGGCT12190.1733799852079422No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGTT11610.16513056835637482No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11310.16086362860556408No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGGCCTTGGACGTTCGGCCAAGGGA11190.1591568527052398No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG11120.15816123343005065No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11090.15773453945496957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11070.15745007680491552No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10790.15346759970415885No Hit
GTCTTTGTCTCCAGGAGAAGTGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTTCTTAGCCTGGTA10150.14436479490242932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10150.14436479490242932No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10100.1436536382772942No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10090.14351140695226716No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9840.13995562382659157No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9660.13739545997610514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9660.13739545997610514No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9620.13682653467599704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9090.12928827444956478No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8720.12402571542356489No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8700.12374125277351085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8690.12359902144848381No Hit
GTGTTAACAGCTTCTTAGCCTGGTACCAACAGAAAGTTGGCCAGGCTCCC8520.12118108892302441No Hit
GAGTGTTAACAGCTTCTTAGCCTGGTACCAACAGAAAGTTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC8230.11705638049724072No Hit
TCTATGATGCATCCAACAGGGCCACTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8150.11591852989702452No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8030.11421175399670024No Hit
GGCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7890.11222051544632189No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7820.11122489617113272No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7610.10823803834556522No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCA7440.10582010582010583No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7310.10397109859475451No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7150.10169539739432214No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGAAGTGGC7080.10069977811913297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.043.85641
GTATCAA17100.041.1085051
TATCAAC18950.036.8871542
TCAACGC19950.035.16824
ATCAACG20200.034.747783
CAACGCA20350.034.4769365
AACGCAG20750.033.812326
AGAGTAC21650.032.24786811
CGCAGAG22150.031.830478
ACGCAGA22100.031.746867
GCAGAGT23100.030.8191939
GACGTGT1500.030.44994562
ACGGGGG4500.029.0466316
CAGAGTA24250.028.79036510
AGTACGG19750.028.73291813
GAGTACG19950.028.27247612
TGTCTAT1800.027.5894666
TACGGGG9600.027.2292815
GTCTATT1900.026.4536767
ACGTGTC1757.2759576E-1226.13033163
Produced by FastQC (version 0.11.5)