FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256782
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA47120.3749258025656001No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCA44060.3505779045212296No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTA43130.3431780531548033No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37580.29901764983903334No Hit
GTGTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCA37250.2963918961283659No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34010.27061176878726784No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30660.24395639020928056No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC28880.22979323383052908No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28720.2285201411223267No Hit
GTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGGGCTGGATCCG27740.22072244828458715No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27710.2204837434017992No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27200.21642576039440412No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27110.21570964574604026No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGG26540.2111742529730693No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25110.19979598689351058No Hit
GGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCAC24960.19860246247957086No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24530.19518102582627694No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24260.19303268188118544No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24230.19279397699839748No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA23960.19064563305330598No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23660.18825858422542652No Hit
CACCTGCACTGTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGG23500.18698549151722413No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23150.18420060121803145No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAC22650.18022218650489902No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTAT21420.17043528631059326No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21210.16876435213107763No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20990.16701384965729935No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20880.1661385984204102No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20830.16574075694909698No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20150.16033011293923688No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20100.15993227146792363No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19630.15619256163757916No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA19320.15372594451543703No Hit
GACTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTGGCGGAGGGACCAAGCTGACCGTC19250.1531689664555985No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19170.1525324201014973No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19100.15197544204165878No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18960.1508614859219817No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18710.1488722785654155No Hit
ACCTATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAGCCAGTTCTCCCTGAAG18660.14847443709410224No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18200.14481429555802042No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17890.14234767843587828No Hit
GTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCG17500.139244514959635No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17250.13725530760306878No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17180.13669832954323025No Hit
GTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC17140.13638005636617967No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17120.13622091977765435No Hit
GTAATAGGTGGTCCCACTATAATAGATACTTCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAGCC17100.13606178318912907No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16990.13518653195223992No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16950.13486825877518932No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16710.13295861971288578No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTT16690.13279948312436046No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16550.1316855270046834No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16490.1312081172391075No Hit
ATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCC16430.1307307074735316No Hit
CCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG16250.12929847817680393No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16090.12802538546860157No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGG16030.12754797570302565No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT15990.12722970252597507No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15930.12675229276039918No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15760.12539963175793417No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCA15610.12420610734399443No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15530.12356956098989323No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15480.12317171951857998No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT15280.12158035363332702No Hit
ATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT15260.12142121704480172No Hit
CCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCT15220.12110294386775114No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA15190.12086423898496318No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT15150.1205459658079126No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14930.11879546333413434No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14890.11847719015708373No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC14700.11696539256609341No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14680.1168062559775681No Hit
GCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCT14680.1168062559775681No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14560.11585143644641634No Hit
CTATTATAGTGGGACCACCTATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAG14530.11561273156362839No Hit
GCTCTGGAGATGCATTGCCAAACCAATATGCTTATTGGTACCAGCAGAAG14450.1149761852095272No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14340.11410093397263806No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA14250.11338481932427422No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14040.11171388514475859No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13890.11052036073081886No Hit
CACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT13790.10972467778819239No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC13780.10964510949392973No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13770.10956554119966708No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13700.10900856313982854No Hit
GAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTG13680.10884942655130325No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13670.1087698582570406No Hit
CTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTG13600.10821288019720206No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13590.1081333119029394No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13550.10781503872588881No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC13540.10773547043162618No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG13460.10709892407752498No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13430.10686021919473704No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13380.10646237772342379No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG13340.1061441045463732No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT13300.1058258313693226No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC13300.1058258313693226No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13100.10423446548406964No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC12930.1028818044816046No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAACAGACAGCAG12930.1028818044816046No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12920.10280223618734197No Hit
GCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTATCACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGT12620.1004151873594625No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12570.10001734588814926No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12570.10001734588814926No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC12570.10001734588814926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)