FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256782
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA45300.36044437300979804No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCA41040.32654827965390976No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTA38970.3100776427415415No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37660.29965419619313455No Hit
GTGTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCA36600.29121995700129377No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33100.2633710540093668No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29420.23408992172071214No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29320.2332942387780856No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC27890.2219159726985269No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27080.21547094086325236No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26890.213959143272262No Hit
GTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGGGCTGGATCCG25970.20663886020009836No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGG25880.2059227455517345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25870.20584317725747184No Hit
GGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCAC25480.20274001378122858No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25320.2014669210730262No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24640.19605627706316608No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22560.17950607185653517No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA22500.1790286620909593No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22210.17672118155734248No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22150.1762437717917666No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21970.1748115424950389No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21900.17425456443520038No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAC21670.17242449366715948No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21260.16916219360239088No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21250.1690826253081282No Hit
CACCTGCACTGTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGG21080.1677299643056632No Hit
GACTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTGGCGGAGGGACCAAGCTGACCGTC20840.16582032524335963No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20580.16375154959253077No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19960.15881831534824656No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19030.15141846398182024No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19010.15125932739329495No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18760.1492701200367287No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18760.1492701200367287No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTAT18600.14799702732852635No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18520.14736048097442517No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18470.14696263950311192No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA18070.14377990773260596No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18040.14354120284981803No Hit
ACCTATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAGCCAGTTCTCCCTGAAG17840.14194983696456506No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17770.14139285890472653No Hit
GTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCG17580.1398810613137362No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17390.13836926372274586No Hit
GTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC17070.1358230783063411No Hit
GTAATAGGTGGTCCCACTATAATAGATACTTCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAGCC16950.13486825877518932No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16920.1346295538924014No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16760.133356461184199No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16620.13224250506452193No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16590.132003800181734No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCA16500.13128768553337014No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16450.13088984406205692No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT16350.13009416111943042No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16290.12961675135385453No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16270.12945761476532924No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA16160.1285823635284401No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC16150.12850279523417746No Hit
CCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG16070.12786624888007625No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16000.1273092708202377No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15890.12643401958334857No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15660.12460394881530767No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15620.12428567563825708No Hit
CCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCT15350.12213733169316555No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15290.12165992192758968No Hit
ATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCC15210.12102337557348848No Hit
ATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT15070.11990941945381141No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15030.11959114627676082No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14970.11911373651118491No Hit
GAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTG14830.11799978039150782No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC14760.11744280233166929No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTT14740.117283665743144No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14720.1171245291546187No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14680.1168062559775681No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGG14670.11672668768330546No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14630.11640841450625487No Hit
GCTCTGGAGATGCATTGCCAAACCAATATGCTTATTGGTACCAGCAGAAG14620.11632884621199222No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14540.11569229985789102No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14420.11473748032673925No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14200.11298697785296098No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14100.11219129491033449No Hit
CTATTATAGTGGGACCACCTATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAG14070.11195259002754654No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA13850.11020208755376827No Hit
GCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCT13840.11012251925950561No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA13750.10940640461114179No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC13670.1087698582570406No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13650.1086107216685153No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13620.10837201678572735No Hit
CTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTG13510.10749676554883822No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT13350.10622367284063584No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13250.10542798989800937No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13190.10495058013243347No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG12990.1033592142471805No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC12970.10320007765865519No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12950.10304094107012991No Hit
GTCCCTCACCTGCACTGTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCT12900.10264309959881665No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT12890.102563531304554No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12690.10097216541930104No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAACAGACAGCAG12650.10065389224225045No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12580.1000969141824119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA200.00742405451.635279
GGTATCA6350.045.660141
GTGGTAT2100.042.7352031
TGGTATC2100.042.6739162
GTATCAA16550.042.5465051
TACGGAG16700.038.5573470
TATCAAC19050.037.09092
ATCAACG19900.035.4783443
TCAACGC20200.034.9389234
CAACGCA20400.034.593635
TCTAGGG400.002608492334.4673922
TATACTG400.00262432734.424885
CAAAACG400.002624839334.4235114
AACGCAG20850.033.8456576
CGCAGAG21950.032.3063478
GACAGGT1500.032.2157671
GGGCAGT4100.031.9912681
AGAGTAC22500.031.51663811
CAGAGTA22650.031.30791910
TCGCTAT450.004669678430.6035524
Produced by FastQC (version 0.11.5)