FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813487
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22250.039.910511
GGTATCA9250.038.698061
ATCAACG24700.035.9495663
CAACGCA25400.035.3653265
TCAACGC25600.034.9545944
TATCAAC25300.034.8249362
AACGCAG26500.033.895256
GTGGTAT3350.032.87781
ACGCAGA27600.032.419667
CGCAGAG28500.031.3958848
AGAGTAC28800.030.95124611
CAGAGTA30750.028.98848310
AGTACGG22150.028.90081413
GCAGAGT30850.028.8927449
TGGTATC3950.028.7533042
GAGTACG23150.027.652412
GCCGTTA1101.1765733E-625.03042215
CCGTTAA1101.1765733E-625.03042216
GTACGGG25450.025.01812714
TACGGGG10550.024.4669415
Produced by FastQC (version 0.11.5)