FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248784
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA250.00897138349.205969
GTATCAA7150.042.38831
GGTATCA2700.038.267221
CAACGCA8250.037.5034835
GTGGTAT1200.037.310541
TATCAAC8150.037.1573522
ATCAACG8150.037.1424063
TCAACGC8300.036.8707734
AACGCAG8600.035.9771776
ACGCAGA9000.034.3781937
AGAGTAC8900.034.3781911
CGCAGAG9250.033.8207058
GCAGAGT9450.032.7411359
CAGAGTA9400.032.54956410
TGGTATC1359.094947E-1230.5891362
GTTAGCC450.004682515330.576833
CGTACAC450.004682515330.576833
GAGTACG7700.029.0205512
AGTACGG7750.028.83332313
CGGGAAC854.525815E-628.31714417
Produced by FastQC (version 0.11.5)