FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802105
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27460.34234919368411865No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT26330.32826126255290766No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22090.27540035282163805No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT21790.2716601941142369No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC21290.2654265962685683No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT20740.2585696386383329No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG19370.24148958054120095No Hit
GTCCCAGGTCCAGTTGGTGCAGTCTGGACTTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGA19090.23799876574762657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16100.2007218506305284No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT15750.1963583321385604No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA15450.19261817343115925No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC15350.19137145386202556No Hit
GTAATAAACTCCGACATCCTCAGGCTCCACCCTGCTGATTTTCAGTGTGA14720.18351712057648312No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG14670.18289376079191627No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA14170.1766601629462477No Hit
GTTTATTACTGCGCGCAAGTTACACAATTTCCTCAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13980.17429139576489364No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG13780.1717979566266262No Hit
GTCTGGACTTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGACTTCTGGTTACTTCTTTTCCTC12900.1608268244182495No Hit
GTAATAAACGCCCGTGTCGTCAGACTTCAGGTTCCTCAGCTCCATGTAGGCCGTGGTCGCGGATATGTCTATGGT12660.15783469745232856No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA12510.155964618098628No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCAGTCCC12310.15347117896036055No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12210.15222445939122684No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12060.15035438003752627No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA11990.14948167633913267No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11990.14948167633913267No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGTTGTTACAATGGT11630.1449934858902513No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT11360.14162734305359026No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA11320.14112865522593673No Hit
GTGATGGGAACACCTACTTGAGTTGGCTTCAGCAGAGGCCGGGCCAGCCTCCAAGACTCCTAATTTATAAGGTTT11140.13888456000149604No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG11080.13813652826001585No Hit
ACATGGAGCTGAGGAACCTGAAGTCTGACGACACGGGCGTTTATTACTGT10980.13688980869088213No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC10960.13664046477705535No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA10930.13626644890631526No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10750.13402235368187457No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10710.1335236658542211No Hit
GTCTATGGTCATGGTGACTCTGTCCTTGACATTCTGTGCATAGTGTGTCACACCATTGTAACAACTGATCCACCC10550.13152891454360713No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT10510.13103022671595363No Hit
GTCCCAGACAGATTCACTGGCAGTGGGGCAGGGACAGATTTCACACTGAA10490.1307808828021269No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT10430.13003285106064666No Hit
GTCCAGGGGCCTGTCGCACCCAGGTCATGTAAAAGGAGGAAAAGAAGTAACCAGAAGTCTTGCAGGAGACCTTCA10390.1295341632329932No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG10100.1259186764825054No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTC10080.12566933256867868No Hit
ATGTAGGCCGTGGTCGCGGATATGTCTATGGTCATGGTGACTCTGTCCTTGACATTCTGTGCATAGTGTGTCACA10070.12554466061176528No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT10040.12517064474102518No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG10030.1250459727841118No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA9950.12404859712880484No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.12342523734423798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9890.1233005653873246No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9860.1229265495165845No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9680.1206824542921438No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCGAAGTTG9640.1201837664644903No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9620.11993442255066357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9600.11968507863683682No Hit
GTCAAGGACAGAGTCACCATGACCATAGACATATCCGCGACCACGGCCTACATGGAGCTGAGGAACCTGAAGT9540.1189370468953566No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG9510.11856303102461647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9500.11843835906770311No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9490.11831368711078974No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9340.11644360775708916No Hit
GGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCAGTCCCAGGTCCAGTTGGTGCAGT9320.11619426384326241No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9290.1158202479725223No Hit
GTGCATAGTGTGTCACACCATTGTAACAACTGATCCACCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCA9190.11457352840338858No Hit
CGTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG9160.11419951253264847No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCA9100.11345148079116824No Hit
GAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCAC8990.11208008926512115No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGTTGTTACAATGGTGTGACACACTATG8990.11208008926512115No Hit
GGCCCCAGACGTCCATGCCATTACCTCGATCTTCACTCCAAAAATCGTGA8970.1118307453512944No Hit
GCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTGTCCACCTTGGTGTTG8950.11158140143746767No Hit
CCATTACCTCGATCTTCACTCCAAAAATCGTGAGATCTCGTACAGTAATA8940.1114567294805543No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGGGG8930.11133205752364092No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8750.10908796229920023No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG8600.10721788294549965No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG8580.10696853903167292No Hit
GTCCTGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTG8540.10646985120401943No Hit
GCCCAGCACCACCTGTGGCAGGACCGTCAGTCTTCCTCTTCCCCCCAAAA8350.10410108402266537No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8330.10385174010883862No Hit
GTGTGTCACACCATTGTAACAACTGATCCACCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGGT8250.10285436445353165No Hit
CACTATGCACAGAATGTCAAGGACAGAGTCACCATGACCATAGACATATC8180.10198166075513805No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAAC8160.1017323168413113No Hit
CTCCACGGCGATGTCGCTGGGGTAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC8120.10123362901365782No Hit
ACCATAGACATATCCGCGACCACGGCCTACATGGAGCTGAGGAACCTGAAGTCTGACGACACGGGCGTTTATTA8040.10023625335835085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)