FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802105
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT30190.37638463792146915No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT24480.3051969505239339No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22580.2815092787103933No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT20820.2595670142936398No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC20580.25657488732771894No Hit
GTCCCAGGTCCAGTTGGTGCAGTCTGGACTTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGA18630.23226385572961147No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG18430.22977041659134403No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT17210.21456043784791268No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA16410.20458668129484295No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15330.1911221099481988No Hit
GTAATAAACTCCGACATCCTCAGGCTCCACCCTGCTGATTTTCAGTGTGA14490.18064966556747558No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA13550.16893050161761863No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG13520.16855648574687854No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC13510.16843181378996516No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13240.16506567095330413No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT13190.16444231116873725No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG13180.16431763921182388No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA13140.1638189513841704No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT13130.163694279427257No Hit
GTTTATTACTGCGCGCAAGTTACACAATTTCCTCAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12890.16070215246133612No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA12770.15920608897837563No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA12480.15559060222788787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12480.15559060222788787No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12060.15035438003752627No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT11880.14811028481308558No Hit
GTAATAAACGCCCGTGTCGTCAGACTTCAGGTTCCTCAGCTCCATGTAGGCCGTGGTCGCGGATATGTCTATGGT11870.14798561285617218No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA11790.14698823720086523No Hit
GTCTGGACTTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGACTTCTGGTTACTTCTTTTCCTC11770.14673889328703846No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11630.1449934858902513No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11350.14150267109667689No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA11310.1410039832690234No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGTTGTTACAATGGTGTGACACACTATG11200.1396325917429763No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGTTGTTACAATGGT11180.13938324782914954No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11020.1373884965185356No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCAGTCCC10990.1370144806477955No Hit
ACATGGAGCTGAGGAACCTGAAGTCTGACGACACGGGCGTTTATTACTGT10550.13152891454360713No Hit
GTCCAGGGGCCTGTCGCACCCAGGTCATGTAAAAGGAGGAAAAGAAGTAACCAGAAGTCTTGCAGGAGACCTTCA10450.13028219497447344No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10340.12891080344842631No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10260.12791342779311937No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10190.12704072409472575No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG10180.1269160521378124No Hit
GTGATGGGAACACCTACTTGAGTTGGCTTCAGCAGAGGCCGGGCCAGCCTCCAAGACTCCTAATTTATAAGGTTT10170.12679138018089903No Hit
GTCCCAGACAGATTCACTGGCAGTGGGGCAGGGACAGATTTCACACTGAA10020.12492130082719843No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTC9800.12217851777510426No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT9680.1206824542921438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9610.1198097505937502No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC9350.11656827971400253No Hit
GGCCCCAGACGTCCATGCCATTACCTCGATCTTCACTCCAAAAATCGTGA9310.11606959188634905No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9260.11544623210178219No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA9260.11544623210178219No Hit
GTCAAGGACAGAGTCACCATGACCATAGACATATCCGCGACCACGGCCTACATGGAGCTGAGGAACCTGAAGT9210.11482287231721533No Hit
GTCTATGGTCATGGTGACTCTGTCCTTGACATTCTGTGCATAGTGTGTCACACCATTGTAACAACTGATCCACCC8990.11208008926512115No Hit
ATGTAGGCCGTGGTCGCGGATATGTCTATGGTCATGGTGACTCTGTCCTTGACATTCTGTGCATAGTGTGTCACA8880.11070869773907406No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGGGG8870.11058402578216069No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCA8860.11045935382524733No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG8850.11033468186833395No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG8840.11021000991142058No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT8810.10983599404068047No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8800.10971132208376709No Hit
GTGCATAGTGTGTCACACCATTGTAACAACTGATCCACCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCA8760.1092126342561136No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8740.10896329034228686No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG8730.1088386183853735No Hit
CCATTACCTCGATCTTCACTCCAAAAATCGTGAGATCTCGTACAGTAATA8680.10821525860080664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8530.10634517924710606No Hit
GGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCAGTCCCAGGTCCAGTTGGTGCAGT8490.10584649141945257No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG8470.10559714750562582No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8440.10522313163488571No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8290.10335305228118513No Hit
GGTCAGGCTGACCTGGTTCTTGGTCATCTCCTCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTG8230.10260502053970491No Hit
GTGTGTCACACCATTGTAACAACTGATCCACCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGGT8200.10223100466896479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.049.725451
GTATCAA11050.040.0699271
CTCGTAT350.00126047639.96803760
GTATTAA459.85777E-538.4351271
TATACTG451.0025906E-438.3249175
CCGCTAT650.001592073638.10959670
TAACGGC400.002481643334.8179951
TTAACGG400.002489312634.79609750
TATCAAC12900.034.537642
TGGTATC1600.034.537642
CCTTTGT11600.033.63335870
GTGGTAT1650.033.5433851
ATCAACG13450.033.3508763
TCAACGC13600.032.7170834
CAACGCA13600.032.463465
AACGCAG13750.032.1093146
CGCGGTA450.004352702831.04504658
ATATTAT450.00440131730.9746652
ATTCTAA450.004562286730.7481021
ACGCAGA14550.030.3438517
Produced by FastQC (version 0.11.5)