FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228322
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT5330.2334422438485998No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC3730.16336577289967677No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC2430.10642864025367682No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT2360.10336279464966144No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT2300.10073492698907682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC653.6379788E-1252.8494873
ACCGTCA200.00680191252.7765858
CCGTCCC305.433911E-447.40163464
GACGTCC306.1623316E-446.20768742
CTCGTAT751.6370905E-1146.10582439
TTAGACG306.2297494E-446.1058239
TTATACA751.8189894E-1145.8028952
GTGGTAT1150.044.816991
GGTATCA4050.044.1161461
TCGTATG856.366463E-1140.6995540
CTTATAC856.91216E-1140.423171
GTATCAA9950.040.0575331
CGCAGAT901.2369128E-1038.2612532
ACGAGAC901.2732926E-1038.18580222
GTATAGG451.02330945E-438.1774371
ATGCCGT952.0190782E-1036.56053544
CGTATGC952.0918378E-1036.44754441
TCTCCGA952.2737368E-1036.1601811
CACGAGA1003.929017E-1034.3672221
ACGGGTA601.3757128E-534.35969516
Produced by FastQC (version 0.11.5)