FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228322
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10750.4708262891880765No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC3530.1546062140310614No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT2390.10467672847995375No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC2320.10161088287593838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA300.00394051360.567770
CAATAAG252.6413397E-454.8637715
TTATACC200.007479212251.5250662
GACGAGT200.00753126251.4347888
GTGGTAT950.047.0672841
TGGTATC950.047.0053252
GGTATCA3300.042.733581
ATATCTC350.001386574939.188416
GTATCAA8100.039.0662881
ATCAACG9750.032.369743
TCAACGC9950.031.7121454
ACGGGTA553.3630957E-431.1794316
TATCAAC10100.030.9490492
CAACGCA10250.030.777245
AACGCAG10400.030.3333386
GCTAACA800.004899665730.28384870
ACGCAGA10600.029.761017
CGCAGAG10750.029.6647158
AGAGTAC11050.028.54902311
GCAGAGT11200.028.472839
Produced by FastQC (version 0.11.5)