FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707659
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT48550.6860648984892441No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45950.6493240388379149No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA38720.5471561868074878No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACT32790.4633587646027253No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30120.4256287279607833No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATT27270.3853550933429802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27180.3840832943550495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27000.3815396963791883No Hit
GTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCAGTGGCCCTAGT24420.345081458725177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24330.34380965973724636No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24260.3428204827466336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23230.3282654498847609No Hit
GAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG22690.3206346559571771No Hit
CTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCT21650.3059383120966454No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21110.29830751816906165No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20490.2895462362522062No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCGCCGCCGAAAGTG19790.27965446634607916No Hit
CTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT19690.27824135635948954No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT19680.27810004536083055No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCAC19520.27583906938228725No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19090.269762696439952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18400.26001223753248387No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTT18310.25874043854455325No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17920.25322930959685386No Hit
CATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA17040.2407939417148655No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16810.23754378874570944No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16440.232315281795328No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16390.23160872680203318No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16300.23033692781410253No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15860.2241192438731084No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCA15720.22214088989188294No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15430.21804287093077312No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15260.21564058395357083No Hit
CACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCGACAG14800.20914027801525875No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14500.20490094805549003No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14180.20037899609840334No Hit
CTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTC13930.19684622113192934No Hit
GGATACCAGTGGCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGG13150.1858239632365306No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGGC13050.184410853249941No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12840.18144332227810286No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12420.1755082603344266No Hit
GTTTATTACTGTCAACAGTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAA12180.17211679636661162No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC11990.1694318873920914No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11900.16816008840416077No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT11760.16618173442293535No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11760.16618173442293535No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11760.16618173442293535No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11740.16589911242561742No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11620.1642033804417099No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11330.16010536148060012No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11280.15939880648730534No Hit
CCTTGGTCCCGCCGCCGAAAGTGAGAGGCCAGTTATTATACTGTTGACAGTAATAAACTGCAAAATCCTCAGA10790.15247456755301636No Hit
GTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGG10710.15134407956374468No Hit
CACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGGCCTCTCACTTT10640.15035490257313197No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCGCCGCCGAAAGTGAGAGGCCAGTT10460.14781130459727074No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10450.14766999359861174No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTCTCCAGCCACC10330.14597426161470425No Hit
GCAGAAGCCTGACCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTT10280.14526770662140948No Hit
GTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCTCCTCATTTATGGTG10280.14526770662140948No Hit
GCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTT10140.14328935264018405No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10060.14215886465091238No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTCTC10040.14187624265359447No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGG9580.13537593671528236No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9570.1352346257166234No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9480.13396282672869275No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9260.1308539847581957No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9230.1304300517622188No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9210.1301474297649009No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9140.1291582527742882No Hit
GAGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAA9070.12816907578367548No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9020.12746252079038067No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9020.12746252079038067No Hit
GGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGT8920.1260494108037911No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8830.12477761181586046No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACG8790.12421236782122463No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8750.1236471238265888No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8700.12294056883329399No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTT8700.12294056883329399No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8620.12181008084402234No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8610.12166876984536339No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8600.1215274588467044No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8600.1215274588467044No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8570.12110352585072753No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8370.11827730587754837No Hit
GAAGAGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGG8360.11813599487888941No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8290.1171468178882767No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8270.11686419589095878No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGC8260.11672288489229983No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8260.11672288489229983No Hit
CCTCATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTT8090.11432059791509754No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8030.11347273192314378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7980.11276617692984897No Hit
CTCCCAGGCTCCTCATTTATGGTGCATCCACTAGGGCCACTGGTATCCCA7870.11121175594460045No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7820.11050520095130564No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7720.10909209096471606No Hit
GTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGGCCTC7700.10880946896739813No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7690.10866815796873917No Hit
GTATCAACGCAGAGTACGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCT7660.1082442249727623No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG7640.1079616029754444No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7630.10782029197678542No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGC7610.10753766997946752No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7560.10683111498617272No Hit
GTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGG7430.10499407200360625No Hit
CTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCTCCT7280.10287440702372189No Hit
GACTGCAGGCTGTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCC7260.10259178502640397No Hit
CCAGTGGCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTC7220.10202654103176813No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7190.10160260803579126No Hit
GGCTTCTGCTGGAACCAGGCTAAGTTGGTGGTGACGCTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC7170.10131998603847334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA100.009193012132.9255770
ACGGGTA150.00240031668.6703916
GTGGTAT2200.057.8477551
GTATCAA14750.042.2079281
GGTATCA5600.036.8528181
TATCAAC17200.036.559472
ATCAACG17150.036.4347763
TCAACGC17400.035.908754
TAGGCAA1151.2940936E-534.6762470
CAACGCA18050.034.613195
AACGCAG18550.033.8652766
ACGCAGA19000.033.06327
CGCAGAG19400.032.7353828
AGAGTAC19500.032.39146811
GCAGAGT19800.031.900699
CCGATTT450.004553798630.75929341
CGGTTCA802.5040954E-630.56604855
GAGTACT5300.028.49864812
CAGAGTA22550.028.16259210
CGTTGGT950.00722374627.98433370
Produced by FastQC (version 0.11.5)