FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895760
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.043.017381
GGTATCA10100.042.331981
TCAACGC30450.036.949324
ATCAACG30850.036.3749543
CAACGCA31050.036.3420835
TATCAAC31250.035.6027642
AACGCAG32250.034.989826
ACGCAGA33700.033.2801487
CGCAGAG34600.032.414488
CAGAGTA35100.031.8547210
AGAGTAC35250.031.81676711
GCAGAGT36900.030.3940649
AGTACGG29450.027.21875213
GAGTACG29500.027.17261712
TGGTATC3650.025.4801582
AGTACTT12950.024.97213413
TATACCG700.001355652824.573645
GAGTACT13350.023.96620412
GTGGTAT3750.023.906191
GTACGGG33900.023.13836714
Produced by FastQC (version 0.11.5)