FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794993
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18620.23421589875634125No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG18070.22729759884678227No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACTGAGGGTGATGTCCCATG17380.2186182771420629No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACTGAGG16950.21320942448549862No Hit
GCATGGGACATCACCCTCAGTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16740.21056789179275792No Hit
GACTATTACTGCTCAGCATGGGACATCACCCTCAGTTCTTGGGTGTTCGG15400.19371239746765065No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15290.19232873748573887No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC14990.18855511935325217No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14130.1777374140401236No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13660.1718254122992278No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG13440.16905809233540423No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13340.1678002196245753No Hit
CCCAAGAACTGAGGGTGATGTCCCATGCTGAGCAGTAATAGTCAGCCTCG12700.15974983427527034No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12340.1552214925162863No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12220.15371204526329163No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAAT12190.15333468345004295No Hit
GTTCTGGGAACAGCAATAATGTTGGCAACGAAGGAGCAGCTTGGCTGCAGCAGCGCCAGGGCCACCCTCCCAAA12130.1525799598235456No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11320.14239119086583152No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11210.14100753088391973No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11150.1402528072574224No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10860.13660497639601857No Hit
CACCTGTTCTGGGAACAGCAATAATGTTGGCAACGAAGGAGCAGCTTGGCTGCAGCAGCGCCAGGGCCACCCTCC10780.13559867822735544No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10760.13534710368518968No Hit
GACTTGAGACAGACCGCCACACTCACCTGTTCTGGGAACAGCAATAATGTTGGCAACGAAGGAGCAGCTTGGCTG10580.13308293280569766No Hit
GTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10550.13270557099244898No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10270.12918352740212807No Hit
GTCTGAGAGTCGAGGACACGGCTGTGTATTATTGTGCGAGAGAGATTGCA9800.12327152566123224No Hit
GTGTATTATTGTGCGAGAGAGATTGCAGGCACCAGTCCTGACTTCTGGGGCCAGGGAATCCTGGTCACTGTCTC9710.12213944022148623No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9590.12062999296849156No Hit
ATAATACACAGCCGTGTCCTCGACTCTCAGACTGCTCATGTGCAAATACA9290.11685637483600485No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9290.11685637483600485No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8990.11308275670351814No Hit
GGGCTGGAGTGGGTGGCACTTATACGGGAGGACGGAAATGAAAAATTCTA8840.11119594763727479No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8800.11069279855294323No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8790.11056701128186035No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8760.11018964946861168No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8740.10993807492644589No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8610.10830284040236832No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8460.10641603133612497No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8230.1035229241012185No Hit
CAATAATACACAGCCGTGTCCTCGACTCTCAGACTGCTCATGTGCAAATA8210.10327134955905272No Hit
GTCCAAGGACTTGAGACAGACCGCCACACTCACCTGTTCTGGGAACAGCAATAATGTTGGCAACGAAGGAGCAG8060.10138454049280936No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8040.10113296595064358No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8010.10075560413739491No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGAATCTCTGAGAGATT8010.10075560413739491No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7970.10025245505306335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.043.9248541
GTATCAA19200.042.160881
GTGGTAT2100.041.007541
TATACAC1600.040.897313
TTATACA1550.039.9946482
TCGTATG1400.037.0700440
CGTATGC1500.036.9286241
TATCAAC22400.036.284832
ATCAACG23050.035.2616123
TCAACGC23100.035.1852844
GGATATA400.002615745934.4463351
AACGCAG23900.034.295746
CAACGCA23700.034.2945185
TATCTCG1550.033.4129436
TGGTATC2750.032.56132
ACGCAGA25550.032.0809447
AGAGTAC25550.031.9441411
CGCAGAG26050.031.4632078
CAGAGTA26100.031.27098710
GTTAGAC450.004638565230.6440331
Produced by FastQC (version 0.11.5)