FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119257
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34820.311099238155312No Hit
GTATTACTGTCACCAGTATCGTGACTCACACACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGG34590.30904430349776685No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22020.19673765721366943No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21280.1901261283154807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20370.18199573467041083No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20250.18092359484908294No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19910.17788586535532053No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19220.17172106138268511No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17740.1584980035863077No Hit
GTGTATTACTGTCACCAGTATCGTGACTCACACACCTTCGGCCAAGGGAC17690.1580512786607544No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17260.15420944430099612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17230.15394140934566414No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16890.15090367985190176No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16690.14911678014968857No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC16680.14902743516457792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15590.1392888317875162No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15200.1358043773682005No Hit
GTAATATATGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA14540.12990760835089707No Hit
GTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14230.12713791381246667No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14120.12615511897624943No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13890.12410018431870427No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13800.12329607945270836No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT13740.1227600095420444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13600.1215091797504952No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13230.11820341530140084No Hit
GTCTCCAGGCACCCTGTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG13180.11775669037584754No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12830.11462961589697451No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12830.11462961589697451No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12730.11373616604586792No Hit
CTCCAGGCACCCTGTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGC12550.11212795631387609No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12440.11114516147765885No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12220.10917957180522435No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA11920.10649922225190463No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG11670.10426559762413816No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11390.10176393804103973No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11250.1005131082494905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.041.9723971
GTATCAA23900.041.0504071
ATCAACG27450.035.7867853
TCAACGC27450.035.5254144
TATCAAC27650.035.435382
CAACGCA28050.034.7624055
AACGCAG29550.032.9978146
CGCAGAG31200.031.252748
ATCGCTA450.004712349330.54639825
AGAGTAC32600.030.22655311
GCAGAGT33550.029.1659879
CAGTTCG11250.027.88222170
CAGAGTA35700.027.69801310
GGCGAAC500.00760807427.69359640
AGTACGG28100.026.88070913
CCTTTGT8200.026.77713670
GAGTACG28450.026.55001312
ACGCAGA36950.026.4822447
TACGGGT2800.025.75394615
TACGGGG14200.023.6984715
Produced by FastQC (version 0.11.5)