FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557650
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.040.0029561
GTATCAA70350.039.9144021
TCAACGC83300.034.119144
TATCAAC83750.033.9358142
ATCAACG83900.033.9159053
CAACGCA85050.033.41715
AACGCAG87350.032.5763556
ACGCAGA93100.030.598737
CGCAGAG94000.030.3057638
AGAGTAC97700.029.194211
TAAGCGC605.056598E-429.09664347
CAGAGTA99000.028.77573210
CTTACGA1056.1259535E-428.15712570
GCAGAGT102500.027.8259689
GAGTACG61900.027.29416712
AGTACGG62050.027.28383813
GTGGTAT12000.026.2214761
TACGGGG28500.025.6824515
GTACGGG66700.025.1258514
TGGTATC13300.022.8865532
Produced by FastQC (version 0.11.5)