FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697462
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGGGGTTACTACTGGACCTGGATCCGCCAGCC31630.4535014093957807No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA21360.3062532439043274No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19910.2854635808115711No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16450.23585514336264915No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15320.2196535438489839No Hit
GTTTATTACTGTGCGAGAGGGGGATATTGTAGTGGTGACACCTGCTTCTCACCGCTCGGCTATGACCACTGGGGC12400.17778746368977807No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11880.17033186037375514No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11560.16574379679466408No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10720.15370012989955006No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10680.1531266219521637No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC10210.14638790357037373No Hit
GTATAACAGTGCCCCTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT10150.14552764164929416No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10090.14466737972821458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9780.14022269313597013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9600.1376419073727314No Hit
AGTCAGGGCATTGCCAACTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGTTCCTAAACTCCTGATCTATG9500.13620813750426547No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9340.13391410571471993No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9140.13104656597778805No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9110.13061643501724823No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8950.12832240322770272No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8470.12144030785906615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8130.11656549030628191No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTGCCAA8080.11584860537204894No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG7980.114414835503583No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT7970.1142714585167364No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTGCCAACTATTTAGCCTGGTATCAGCAAAAA7840.11240755768773066No Hit
GGTCATAGCCGAGCGGTGAGAAGCAGGTGTCACCACTACAATATCCCCCTCTCGCACAGTAATAAACAGCCGTGT7770.11140391877980449No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCACTGCTGTAGCTGCAC7740.1109737878192647No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7690.11025690288503173No Hit
CTACAATATCCCCCTCTCGCACAGTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAA7670.10997014891133855No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT7600.10896651000341237No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT7590.10882313301656577No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGGGGTTACTACTGGA7560.10839300205602599No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG7510.10767611712179301No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG7510.10767611712179301No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT7480.10724598616125322No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC7400.10609897026648046No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7370.10566883930594069No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG7290.10452182341116792No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT7130.1022277916216224No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCATAGCCGA7090.10165428367423601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)