FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125016
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGG28080.24959645018381962No Hit
GATTACTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT24670.21928577015793552No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24100.21421917554950332No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAG23850.21199698493176983No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAAT22420.19928605459833462No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTG21820.19395279711577434No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20680.1838196078989099No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19770.17573083405036016No Hit
GAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAG19050.16933092507128786No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18450.16399766758872764No Hit
CTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATT18250.16221991509454087No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17310.15386447837186315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16960.15075341150703633No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC16540.14702013126924418No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16460.14630903027156947No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15570.13839803167243842No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15240.1354647400570303No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAG14190.12613153946254985No Hit
CTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGT13800.1226649220988857No Hit
CTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC13310.11830942848812817No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13030.1158205749962667No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12890.11457614825033599No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12770.11350949675382395No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12630.11226507000789324No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12240.10879845264422905No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12130.10782068877242634No Hit
TTTCTGGGGTCCAGGCTGAGGACGAGGCTGATTACTACTGCTCCTCGTAC12080.10737625064887966No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11770.10462073428289019No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11760.10453184665818087No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11690.10390963328521549No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11580.10293186941341277No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11540.10257631891457544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21300.041.609571
TATCAAC24200.036.335062
ATCAACG25000.035.172343
TCAACGC25100.035.032214
TATCGAT400.002536440534.66450542
CAACGCA25500.034.6179125
AACGCAG25650.034.5499046
TCGCGAA553.2208697E-431.4233236
AGAGTAC28350.031.25665911
CGCAGAG28700.030.8741078
ACGCAGA29500.030.0368427
CAGAGTA29800.029.73445910
GCAGAGT30050.029.601829
GTATAAA953.2153002E-729.0406361
CTACTAG850.006267661728.81311270
AGTACGG27500.026.58065413
GAGTACG28050.026.05946512
TCGATTA700.001291962324.77807644
ACGGGGA5900.024.54590616
TAGTTCG1001.571335E-524.134667
Produced by FastQC (version 0.11.5)