FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125016
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGG24890.22124129790154096No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24260.21564137754485269No Hit
GATTACTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT24240.21546360229543404No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTG22570.20061936896897467No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAG21400.1902195168779822No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAAT21190.18835287675908607No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20150.179108563789315No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19120.16995313844425322No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18760.16675318395471708No Hit
GAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAG18190.16168658934628485No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17000.1511089620058737No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16490.1465756931456975No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAG16010.14230908715964927No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC15670.13928690791953183No Hit
CTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATT15660.13919802029482248No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15400.1368869420523797No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15250.13555362768173962No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14500.12888705582853932No Hit
CTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGT14310.1271981909590619No Hit
CTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC13550.12044273148115227No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12410.11030954226428781No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCT12220.10862067739481039No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12110.10764291352300767No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12090.10746513827358899No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11500.1022207684157381No Hit
TTTCTGGGGTCCAGGCTGAGGACGAGGCTGATTACTACTGCTCCTCGTAC11500.1022207684157381No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11420.10150966741806339No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11330.10070967879567934No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11290.100354128296842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)