FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178037
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18880.16026661301809705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40400.044.5533451
GGTATCA15400.039.9266321
TATCAAC45100.039.6110042
TCAACGC46150.038.523534
ATCAACG46550.038.3527033
CAACGCA46650.038.0323565
AACGCAG48100.036.885856
ACGCAGA51400.034.5843287
CGCAGAG52600.033.9255648
AGAGTAC52750.033.6992311
CAGAGTA54100.032.9849310
GCAGAGT55500.032.2763069
GAGTACG38200.030.754312
AGTACGG38550.030.65277513
GTGGTAT5550.030.3272821
GTACGGG42100.027.66125914
TACGGGG17050.026.1163515
TACGGGA13350.025.40080315
TACGGGT8350.025.02285815
AGTACTT24250.024.71732113
Produced by FastQC (version 0.11.5)