FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467677
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15480.330997675746295No Hit
GTAATATACGGCCGTGTCCGCAACGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTGTTGGACGTGTCTGCCGA11160.2386262313519801No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG11160.2386262313519801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10680.22836273753038955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9510.20334547134026262No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9320.19928283836921634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9230.1973584332776681No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA9230.1973584332776681No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA8950.19137139521507365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8620.18431524321273016No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8390.17939731908988468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8250.17640380005858744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8120.17362410381524No Hit
GCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGG7750.1657126606610973No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA7670.1640020783574989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7490.16015326817440242No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCC7420.1586565086587538No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7100.1518141794443601No Hit
CAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA6890.14732390089741426No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6880.14711007810946444No Hit
GTATATTACTGTGCGAGACTCGATAGTGGAAACGATCGACACGGCTATTATTACTACTACCACGGTTTGGACGT6780.14497185022996642No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6700.14326126792636798No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6630.14176450841071936No Hit
GTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCAT6560.14026774889507074No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6280.13428071083247628No Hit
CCATAATGCTGACAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCT6210.13278395131682766No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6090.13021807786143No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5980.12786602719398216No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5970.12765220440603237No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5870.12551397652653434No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5790.12380339422293592No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5680.12145134355548808No Hit
GTCCCAGACCCGCTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCG5610.11995458403983945No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5600.11974076125188966No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGAGGTGAACCACCATAATG5590.11952693846393986No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCGGTATATTACTGTCAGCATTATGGTGGTTCA5560.11888547010009046No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5510.11781635616034142No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5400.1154643054928936No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5380.115036659916994No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5300.11332607761339558No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5110.10926344464234931No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5030.1075528623387509No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG4950.10584228003515248No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG4820.10306258379180504No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACC4710.1007105331243572No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT4710.1007105331243572No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4710.1007105331243572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)