FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993384
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36430.3667262609423949No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33700.33924444122313224No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32120.32333921222810114No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAAGGGGGGTG31700.3191112399635992No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30190.30391067301265173No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28940.29132742222544356No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25180.25347700385752137No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24870.2503563576622937No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATT24800.24965169561821007No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24230.24391373325924315No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC23920.24079308706401553No Hit
CTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGTCTCCTGGTATCAACAACACCCAGGCAACGT23630.23787377288138323No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23100.23253847454760695No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23020.23173314649722565No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG21750.21894856369742213No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21610.21753923960925484No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGTCTCCTGGTATCAACAACA21080.21220394127547856No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAAGGGGGGTGA21010.21149927923139492No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGA20940.21079461718731124No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20110.20243933866460503No Hit
CCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19380.19509072020487545No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19360.19488938819228013No Hit
GTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTTCTGTCTTCGGAA18400.18522545158770426No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC18290.18411812551842993No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18080.18200413938617896No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17070.17183687275011475No Hit
TCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTT16690.16801156451080348No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15800.15905228995031126No Hit
TCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACTAGAGACC15240.153414993597642No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14900.14999234938352138No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14750.1484823592890564No Hit
CTCTCGGGGCGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC14660.1475763652323774No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13960.14052974479154084No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC13750.13841575865928987No Hit
GACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13230.13318112633181126No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13090.13177180224364396No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12970.13056381016807198No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12910.12995981413028598No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACT12840.1292551520862023No Hit
ATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGG12730.128147826016928No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCA12690.12774516199173733No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12540.12623517189727235No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12420.12502717982170036No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12280.12361785573353305No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG12110.12190653362647275No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12060.12140320359498441No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12020.12100053956979376No Hit
GGTATCAACAACACCCAGGCAACGTCCCCAAAGTCATCATTTATGATGTT12010.12089987356349609No Hit
TTTATAACCAGAAGCATCACCCCCCTTTCTGTCTTCGGAACTGGGACCAA11930.12009454551311477No Hit
GATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTTCTGT11810.11888655343754279No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11430.1150612451982315No Hit
GGTTATAAATGAGCTGCAATAATAATCAGCCTCGTCCTCAGACTGGAGCC11370.1144572491604455No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11220.11294725906598052No Hit
GACCATCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCC11160.11234326302819453No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10850.10922261683296691No Hit
CTTTGGGGACGTTGCCTGGGTGTTGTTGATACCAGGAGACATGCCTTTGA10770.10841728878258558No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10650.1072092967070136No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10580.10650463466292995No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10420.1048939785621673No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10410.10479331255586963No Hit
CAACAACACCCAGGCAACGTCCCCAAAGTCATCATTTATGATGTTGATAATCGGCCCTCAGGGGTCTCTAGTCG10130.10197466437953501No Hit
CCCCTGAGGGCCGATTATCAACATCATAAATGATGACTTTGGGGACGTTGCCTGGGTGTTGTTGATACCAGGAGA9990.10056534029136768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1600.045.46595441
TCGTATG1650.044.0591940
GTGGTAT2450.040.753551
AACGCGA350.001361979839.34429612
TCTCGTA1800.038.42352738
ATCTCGT1800.038.3963537
CTCGTAT1850.037.40396539
TATGCCG1950.037.36216743
AATCTCG1900.036.3552836
ATGCCGT2050.035.56307244
CACGAGA2100.034.4314621
ACGAGAC2100.034.4314622
AAACGCG400.002623548734.42625811
TGCCGTC2300.033.2322345
ATCAACG24650.033.101153
CCCACGA2300.032.93443719
CAACGCA25000.032.6377375
GTAAGAC851.23051E-732.4028173
AACGCAG25650.031.810666
TATACAC2600.031.7796843
Produced by FastQC (version 0.11.5)