FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993384
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35100.3533376821048054No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34490.3471970557206478No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA29140.29334074235139684No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAAGGGGGGTG29110.2930387443325039No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26380.2655569246132412No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25130.25297367382603303No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC25110.25277234181343766No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24780.24945036360561473No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24760.24924903159301942No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24580.24743704347966144No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23630.23787377288138323No Hit
CTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGTCTCCTGGTATCAACAACACCCAGGCAACGT22790.22941782835237934No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATT22720.2287131663082957No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22380.22529052209417505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20930.21069395118101358No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCGTTGACATTGGTGATCAAAGGCATGTCTCCTGGTATCAACAACA19780.19911736045678208No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19370.1949900541985778No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19340.1946880561796848No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGA19090.19217140602224317No Hit
GTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTTCTGTCTTCGGAA19030.19156740998445718No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18720.18844676378922953No Hit
CCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18720.18844676378922953No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18580.18703743970106224No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAAGGGGGGTGA17780.178984159197249No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17170.17284353281309142No Hit
TCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTT16010.16116627608256223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15620.15724030183695328No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15440.1554283137235953No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15410.1551263157047023No Hit
GACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15320.15422032164802332No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15130.1523076675283677No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14820.14918702133314005No Hit
CTCTCGGGGCGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC14220.14314706095528013No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13930.14022774677264785No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13900.13992574875375485No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC13600.1369057685648249No Hit
TCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACTAGAGACC13590.1368051025585272No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12320.12402051975872372No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12250.12331585771464006No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12030.12110120557609141No Hit
ATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGG12000.12079920755719842No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11990.12069854155090076No Hit
GGTTATAAATGAGCTGCAATAATAATCAGCCTCGTCCTCAGACTGGAGCC11910.11989321350051943No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACT11790.11868522142494745No Hit
GGTATCAACAACACCCAGGCAACGTCCCCAAAGTCATCATTTATGATGTT11770.11848388941235212No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11710.11787989337456613No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11570.11647056928639882No Hit
GATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCCCCTTTCTGT11470.11546390922342216No Hit
GACCATCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATTTATAACCAGAAGCATCACCCC11230.11304792507227819No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCA11210.11284659305968286No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC11190.11264526104708752No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11080.11153793497781321No Hit
TTTATAACCAGAAGCATCACCCCCCTTTCTGTCTTCGGAACTGGGACCAA10920.10992727887705056No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10910.1098266128707529No Hit
CAACAACACCCAGGCAACGTCCCCAAAGTCATCATTTATGATGTTGATAATCGGCCCTCAGGGGTCTCTAGTCG10870.10942394884556224No Hit
GCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10680.10751129472590659No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT10660.10730996271331127No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10460.10529664258735796No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10450.1051959765810603No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10450.1051959765810603No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10440.10509531057476262No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10110.10177333236693967No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACT10020.10086733831026068No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9990.10056534029136768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA200.007437974751.61000426
GTGGTAT2550.041.8677941
ATCAACG23350.037.2612573
CAACGCA23750.036.763695
AACGCAG24500.035.7785766
AGAGTAC26600.033.21241811
ACGCAGA26650.033.0211147
CGCAGAG26850.032.775158
CAGAGTA27650.031.95118510
GCAGAGT28200.031.2061259
CGTCTAA450.00438812730.9943850
AGTACGG21150.028.28055213
ACGCGCC500.00784889727.5170224
GAGTACG21750.027.50039712
TCAACGC33800.026.2419264
GAGTACT10850.025.9796412
AGTACTT11100.025.3945113
TAACGGC700.001330458724.653236
TACGGGG15250.024.572515
GTACTTT11500.024.51122314
Produced by FastQC (version 0.11.5)