FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985095
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33950.043.7702831
TATCAAC38800.038.1216852
TCAACGC38850.038.0726244
ATCAACG39050.037.8776323
CAACGCA39900.036.8982935
AACGCAG40650.036.1328936
CGACGTC2500.035.8487130
TCGTATG2450.035.3532140
TCGACGT2550.035.13506729
GGTATCA14150.034.7628171
ACGCAGA43300.033.9198047
CGCAGAG43350.033.801338
ACTCGAC2700.033.15951527
AGAGTAC44300.032.76589611
CGTATGC2650.032.71515341
GCAGAGT45450.032.2395559
CAGAGTA45450.032.23955510
CTCGACG2900.030.87735728
GTGGTAT4250.030.756011
TATACAC3400.030.351333
Produced by FastQC (version 0.11.5)