FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985095
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25350.2573355869230886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35800.042.696471
GGTATCA14700.041.4522361
TATCAAC41750.036.308732
ATCAACG41800.036.1334533
TCAACGC41600.036.1274644
CAACGCA43150.034.8279535
AACGCAG43550.034.5080646
ACGCAGA44950.033.5096177
CGCAGAG45450.033.4429448
AGAGTAC47350.032.24591411
CAGAGTA48300.031.61167710
TTGTCGC553.2206505E-431.42318537
GCAGAGT48600.031.2753439
GTGGTAT4350.030.8650781
AGTACGG34250.029.15191313
GAGTACG34500.028.94066612
TAACGGC1555.0931703E-1126.73851436
GTACGGG37950.026.21928614
TACGGGA16350.026.02583915
TTAACGG1607.8216544E-1125.88177735
Produced by FastQC (version 0.11.5)