FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881281
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58230.6607427142988446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43620.4949613119992375No Hit
GTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG43200.4901955222000701No Hit
CTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT37040.4202972718122823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35340.40100717024422405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34950.3965817940021401No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34630.3929507160599173No Hit
GTTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACACT34140.38739062796088874No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34110.3870502144038054No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGA33570.3809227703763045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32880.3730932585633867No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCA32680.3708238348494975No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31670.35936324509435696No Hit
GTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC28440.32271205211504617No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT28030.3180597335015733No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27770.3151094826735173No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA26160.29684062177670917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26010.2951385539912922No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25550.289918879449347No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25280.2868551574355966No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24780.28118159815087357No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24160.274146384637817No Hit
GTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGATCTCTGGTGC24100.27346555752365026No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA23760.26960753721003855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23400.26552257452503797No Hit
GACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAA23400.26552257452503797No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT23010.261097198282954No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22870.2595086016832316No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAA22710.2576930627121202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21880.24827495429947996No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21560.24464387635725723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21340.2421475102719791No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21120.23965114418670097No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA21100.23942420181531204No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20870.2368143645443394No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACAC20680.23465841201614468No Hit
GCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATT20140.2285309679886438No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20090.22796361206017152No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18900.21446054096253067No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18890.21434706977683624No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18860.21400665621975284No Hit
GGCCAAAAGTGTACGGAGTATTATAATATTGCTGACAGTAATAAACTGCC18190.20640408677822397No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18040.20470201899280707No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17770.2016382969790566No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17460.19812069022252832No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGGAGTTTAGGAGGCTGCCCTGGTCTCTGCTGGTA17410.19755333429405603No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACACCTCCCA17060.19358184279474994No Hit
GCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTG16550.1877948123243324No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16520.18745439876724904No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAA16510.18734092758155457No Hit
CAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTAC16450.18666010046738782No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAA15940.1808730699969703No Hit
ATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCAT15680.17792281916891434No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA15540.1763342225691919No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15290.17349744292683036No Hit
CTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTATTGACCCAGTCTCCAGACTCCCTGGCTGTGT15290.17349744292683036No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGAT15260.173157029369747No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCACTTCTCTGTT15190.17236273106988576No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT14980.1699798361703021No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14970.16986636498460764No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14890.16895859549905196No Hit
GTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACACCTCCCACAGTAAGAACTA14510.16464669044266245No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14500.16453321925696798No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14220.16135602605752308No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGC14120.16022131420057847No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13920.15795189048668926No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13900.15772494811530036No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13860.1572710633725225No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13820.15681717862974465No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13550.1537534566159942No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13440.15250527357335517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGCTTGGTCC13440.15250527357335517No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13430.15239180238766067No Hit
GGTCTTCACTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTATTGACCCAGTCTCCAGACTCC13080.14842031088835458No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13060.14819336851696563No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC12960.14705865666002105No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12680.14388146346057615No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12500.14183898211807586No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12240.13888873129001986No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12150.1378674906187697No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12150.1378674906187697No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12050.13673277876182513No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11960.13571153809057498No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11950.13559806690488052No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11920.13525765334779713No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11860.13457682623363038No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11750.1333286431909913No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11750.1333286431909913No Hit
AGATGGTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTATTGA11680.13253434489113008No Hit
GGATCTCTGGTGCCTACGGGGACATCGTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11580.13139963303418545No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11450.12992450762015748No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11300.12822243983474058No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11070.12561260256376797No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG10940.12413747714973998No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAG10660.12096028395029508No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10610.12039292802182278No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACA10580.12005251446473938No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10420.11823697549362804No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGGAGTTTAGGAGGCTGCCCTGGT10120.11483283992279421No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10050.11403854162293298No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10010.11358465688015515No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10000.11347118569446067No Hit
CCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT9980.11324424332307176No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATC9930.11267688739459945No Hit
CTGCAGGTCCAGCCAGAGTGTTTTATACACCTCCCACAGTAAGAACTACT9910.11244994502321053No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9730.11040746368071024No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9730.11040746368071024No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC9720.11029399249501577No Hit
CTACGGGGACATCGTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTG9720.11029399249501577No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCC9710.11018052130932132No Hit
GGTCCAGCCAGAGTGTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGGCAGC9640.10938622300946009No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9620.10915928063807118No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9550.10836498233820993No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTG9420.10688985692418197No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9340.10598208743862628No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG9240.10484737558168168No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATT9080.10303183661057029No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC9060.10280489423918138No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8880.10076241289668109No Hit
CCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGG8860.10053547052529216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA200.007326124251.8076837
GTGGTAT1800.044.032281
GGTATCA5950.042.8578761
GTATCAA15800.041.0030981
AGTTCGC602.9413422E-740.1942638
CTCGCTA350.001353832839.39185323
TATCAAC16900.037.922142
GTTCGCT655.508773E-737.1023989
TGGTATC2200.036.0223242
TCAACGC17800.036.0047264
CAACGCA17950.035.703855
ATCAACG17950.035.703853
TAGTTCG601.35499085E-534.4522257
AACGCAG18550.034.3632666
ATAGTAG851.2214514E-732.4293023
AGTCTTA751.6785962E-632.1754927
CGCAGAG20200.031.5527848
TGTTAGA9950.031.0200470
CCCGGTT450.00444176130.91714551
TATAGTA902.016659E-730.6276782
Produced by FastQC (version 0.11.5)