FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178068
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19800.16807179212065856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29750.041.3312031
GGTATCA12150.040.2539861
TATCAAC33500.036.471032
ATCAACG34800.034.885613
TCAACGC35300.034.5716484
CAACGCA35900.033.989525
AACGCAG36600.033.621196
GTGGTAT4450.031.7336941
ACGCAGA39450.031.1051547
CGCAGAG40500.030.2987258
AGAGTAC40700.030.23428711
CAGAGTA42150.029.194210
CGTCTAA605.299172E-428.86303140
GCAGAGT43700.028.2373669
AGTACGG32550.026.18857413
TGGTATC5000.026.1564062
GAGTACG33850.025.08126312
TTAACGG1502.8678187E-823.01463335
GAGTACT16950.022.91500312
AGTACTT17200.022.38209313
Produced by FastQC (version 0.11.5)