FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862361
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27530.31923985430695495No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA27440.3181962078526278No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22540.26137545645037286No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20660.23957484162665058No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18430.21371560170276716No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16880.19574169054491103No Hit
TAGTAATATCGTCCACTCCAAAAATTGCCTTGATCTTTCGCACAGTAATATATGGCCGTGTCCTCGGCTCTCAGG15880.1841456188301651No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15150.17568048647840057No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT13540.15701081101765965No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13300.15422775380612064No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13280.1539958323718257No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTGACACCTGTGCCATGATCTGGGTCCGCCAGGCTCCAGGGAAAGGG12870.1492414429687799No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGG12670.1469222286258307No Hit
GCCCTGGGATTTCCAGGTGTTCTCATTTGGTGATCAGGACTGAACAGAGA12620.14634242504009343No Hit
ATATATTACTGTGCGAAAGATCAAGGCAATTTTTGGAGTGGACGATATTACTATTGGTACTATGGTCTGGACGT12410.14390724997999677No Hit
CCCCAGCACTCAGGCTGCCATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTGCGGTGATGC12170.14112419276845775No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12080.14008054631413064No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11940.13845709627406622No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT11510.13347078543672544No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11350.13161541396236612No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC11040.12802063173079486No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10830.12558545667069823No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10640.1233822030448965No Hit
GTATCAACGCAGAGTACGGGAGTTCTGAGAGAGGAGCCCAGCCCTGGGAT10580.12268643874201175No Hit
GTACTAAGCCTCCCCCCGACTCCAACACCTGCATCTCACACTGGACACTT10380.12036722439906257No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG10370.12025126368191512No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10360.12013530296476765No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT10350.1200193422476202No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAGACCATAGTACCAATAGTAATATCGTCCACT10100.11712032431893371No Hit
GTATGTGCTACTACCACTGCCACTAATACCTGAGACCCACTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGAT10040.11642456001604896No Hit
ATATTACTATTGGTACTATGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATC9990.11584475643031168No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC9870.11445322782454218No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTTTTTTAAAAAGTGTCCA9830.11398938495595233No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9810.11375746352165741No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9660.11201805276444551No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC9580.11109036702726585No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9340.10830730981572682No Hit
GTGTATCTGCAAATGAATAGCCTGAGAGCCGAGGACACGGCCATATATTA9200.1066838597756624No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9140.10598809547277764No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9000.10436464543271322No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGGCTGCCATCCCATGTT8890.10308907754409116No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATA8840.10250927395835388No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC8840.10250927395835388No Hit
GTGTGAGATGCAGGTGTTGGAGTCGGGGGGAGGCTTAGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC8790.10192947037261657No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGATCATGGCACAGGTGT8780.10181350965546912No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8680.10065390248399453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.048.845071
CGTATGC1150.048.0952541
TGGTATC1450.047.229982
TCGTATG1200.046.0535440
ATATCGC306.548601E-445.655656
TTATACA1450.042.5069852
TATACAC1450.042.5069853
GTATCAA16850.041.8695871
GTGGTAT1750.041.0972331
CGAGACG1550.039.764623
GACGGAC1500.038.8208126
CTTATAC1500.038.8140531
TCTCGTA1250.038.6172138
ATCTCGT1250.038.5811737
GTACTAA2400.038.5286561
TATCAAC19100.037.289432
TATCTCG1400.036.8757436
CCGTCTT1550.035.9296547
ATCAACG20100.035.604593
CAACGCA20550.034.8249325
Produced by FastQC (version 0.11.5)