FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862361
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28580.3314157296074382No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA24260.28132069979973584No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22680.2629989064904373No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17900.20756968369395182No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17830.20675795867391963No Hit
TAGTAATATCGTCCACTCCAAAAATTGCCTTGATCTTTCGCACAGTAATATATGGCCGTGTCCTCGGCTCTCAGG15500.17973911157856165No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15120.1753326043269582No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15100.17510068289266328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14260.1653599826522767No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14140.1639684540465072No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13000.15074893229169686No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12860.14912548225163244No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTGACACCTGTGCCATGATCTGGGTCCGCCAGGCTCCAGGGAAAGGG12720.14750203221156802No Hit
ATATATTACTGTGCGAAAGATCAAGGCAATTTTTGGAGTGGACGATATTACTATTGGTACTATGGTCTGGACGT12530.1452987785857663No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC11990.1390368998598035No Hit
GCCCTGGGATTTCCAGGTGTTCTCATTTGGTGATCAGGACTGAACAGAGA11980.13892093914265605No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGG11470.1330069425681356No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC10930.12674506384217282No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT10720.12430988878207617No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10700.12407796734778126No Hit
GTATCAACGCAGAGTACGGGAGTTCTGAGAGAGGAGCCCAGCCCTGGGAT10650.12349816376204398No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10640.1233822030448965No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10530.12210663515627447No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10380.12036722439906257No Hit
GTATGTGCTACTACCACTGCCACTAATACCTGAGACCCACTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGAT10370.12025126368191512No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9990.11584475643031168No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9820.11387342423880487No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG9800.11364150280450995No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG9800.11364150280450995No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9750.11306169921877264No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9700.11248189563303536No Hit
GTACTAAGCCTCCCCCCGACTCCAACACCTGCATCTCACACTGGACACTT9690.11236593491588788No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC9680.11224997419874043No Hit
CCCCAGCACTCAGGCTGCCATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTGCGGTGATGC9630.11167017061300313No Hit
ATATTACTATTGGTACTATGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATC9570.11097440631011837No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC9540.110626524158676No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC9300.10784346694713699No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9250.10726366336139968No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9200.1066838597756624No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9110.10564021332133526No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9110.10564021332133526No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGATCATGGCACAGGTGT9010.10448060614986067No Hit
GTGTATCTGCAAATGAATAGCCTGAGAGCCGAGGACACGGCCATATATTA8830.1023933132412064No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA8800.10204543108976404No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8790.10192947037261657No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTTTTTTAAAAAGTGTCCA8690.10076986320114198No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATA8670.10053794176684706No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8650.10030602033255215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.046.6995931
GTATCAA16950.043.843431
TGGTATC2400.042.750792
GGTATCA6200.041.427061
GTACTAA2100.040.769481
CGATTGT650.001187580840.4513670
GTCTTAC350.001397404539.1387061
TATCAAC19250.038.3757742
ATCAACG20450.036.090373
TCAACGC20850.035.3836174
CGTGTTA400.00241016435.02631452
CAACGCA21450.034.3918725
GTATTAC400.002691617234.246371
TATAGTG400.002727874634.153045
AACGCAG21800.033.9963766
AGTACTT4850.032.39257413
CGCAGAG22900.032.363368
AGAGTAC22700.032.1971411
CAGAGTA22750.032.12637710
CTAAGCC2450.032.06394
Produced by FastQC (version 0.11.5)