FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083682
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22160.20448803246708908No Hit
CTCCTGTACAGCCCCTGGATTCACCCTCAACAACTTTGGCATGCACTGGGCCCGCCAGGCTCCAGGCAAGGGG21310.19664440306289113No Hit
GTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT19540.18031119830356138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16270.15013629459564706No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16160.14912123667275087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15530.14330772311434536No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15460.1426617771634114No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15330.1414621632545341No Hit
CTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC14940.1378633215279021No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13810.1274359083199684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13430.123929344586327No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13010.12005366888072332No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12650.11673166113306303No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12000.11073359158867639No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11990.11064131359568583No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11490.10602741394615764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11270.10399729810036523No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA10880.10039845637373325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10840.100029344401771No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10840.100029344401771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21000.040.6435661
TCAACGC24050.035.0848664
ATCAACG24100.035.0379453
TATCAAC24150.035.0041962
CAACGCA24350.034.6510125
AACGCAG24350.034.6494146
AGAGTAC25550.033.15682211
GTGGTAT3800.032.736561
CGCAGAG26200.032.3342328
CAGAGTA26850.031.55146610
ACGCAGA27250.030.961957
GCAGAGT28100.030.3930389
AGTACGG24800.029.16059313
GAGTACG25400.028.6073412
AATACGC500.007801959327.551095
GTACGGG27200.026.4609914
TACGGGG12300.026.3191315
TACGGGC3850.025.04644415
TACGGGA10400.023.18000215
TACGGGT2850.022.9592415
Produced by FastQC (version 0.11.5)