FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294069
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54840.4237795666227999No Hit
GTATTATTGTCTAATGTATGGCAGCTTTCCCAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA39840.3078661184218152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39810.3076342915254133No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC34280.2649008669553169No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32440.2506821506426628No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32400.25037304811412686No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31920.24666381777169533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30320.23429971663025698No Hit
GTCTGGGACAGACTTCACTCTCACAATCAGCAGACTGGAGGCTGAGGATG30150.23298603088397915No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29880.2308995888163614No Hit
ATAATACACTGCAACATCCTCAGCCTCCAGTCTGCTGATTGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29710.22958590307008359No Hit
GTGTATTATTGTCTAATGTATGGCAGCTTTCCCAGGTGGACGTTCGGCCA29300.22641760215259002No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28150.21753090445718118No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT26570.20532135458001083No Hit
CTACATAGCCTGGTACCAGTTGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGCAGGGCCA25950.20053026538770347No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25210.19481186860978822No Hit
CAATAATACACTGCAACATCCTCAGCCTCCAGTCTGCTGATTGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23810.1839932801110296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23560.1820613893076799No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23450.181211357354206No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23320.18020677413646413No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGACTAGTCTCTACATAGCCTGGTACC23230.17951129344725822No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22580.17448837735854889No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGACTAGT22360.17278831345160112No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22120.17093369828038535No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21940.16954273690197352No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT21800.1684608780520977No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21580.1667608141451499No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21360.16506075023820213No Hit
GCCTGGTACCAGTTGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATC21070.16281975690631642No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20630.15941962909242088No Hit
GAGTGTGACTAGTCTCTACATAGCCTGGTACCAGTTGAAACCTGGCCAGG20150.15571039874998938No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC19850.1533921297859697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19790.15292847599316575No Hit
GTTGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT19720.1523875465682278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC18990.14674642542244656No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18780.1451236371476328No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18600.14373267576922097No Hit
GTCTAATGTATGGCAGCTTTCCCAGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTG18040.13940524036971752No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17820.13770517646276975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17790.13747334956636778No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17450.13484597807381216No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17440.13476870244167816No Hit
GTCTCTACATAGCCTGGTACCAGTTGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGCAGGG17270.13345501669540033No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16210.1252637996891974No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15790.12201822313956984No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15740.1216318449788999No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15630.12078181302542601No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15480.11962267854341616No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15140.1169953070508605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14940.1154497944081807No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14830.11459976245470684No Hit
CTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTCTTGACGCAGTC14590.11274514728349105No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14590.11274514728349105No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14500.11204966659428515No Hit
ATGTAGAGACTAGTCACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTG14470.11181783969788318No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14310.11058142958373936No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13850.10702675050557582No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13850.10702675050557582No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13840.10694947487344182No Hit
GTCAGAGTGTGACTAGTCTCTACATAGCCTGGTACCAGTTGAAACCTGGC13680.105713064759298No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13580.1049403084379581No Hit
ACATTAGACAATAATACACTGCAACATCCTCAGCCTCCAGTCTGCTGATTGTGAGAGTGAAGTCTGTCCCAGACC13440.10385844958808224No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13340.10308569326674234No Hit
CCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT13280.1026220394739384No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTCTTGACGCAGTCTC13020.10061287303845468No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13000.10045832177418669No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13000.10045832177418669No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12960.10014921924565075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)