FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454887
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCA19780.43483326628371444No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC19630.43153574404192696No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17140.3767968748282541No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13410.29479848841580436No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12480.27435385051672173No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC12430.2732546764361259No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12110.26621996232031253No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAG12040.26468111860747834No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11650.2561075607788308No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11260.24753400295018324No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGG10690.23500341843139066No Hit
GTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA10550.23192573100572228No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC10410.22884804358005395No Hit
GTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCG10270.2257703561543856No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10160.22335217317707473No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10150.22313233836095558No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9870.21697696350961884No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9670.2125802671872355No Hit
CCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG9520.209282744945448No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8810.19367447300098706No Hit
GACTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGG8560.18817860259800787No Hit
CATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCT8550.1879587677818887No Hit
GGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT8300.1824628973789095No Hit
ATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTT8140.1789455403210028No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7980.17542818326309612No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7800.1714711565729511No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAG7680.16883313877952105No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7590.16685462543444854No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7510.1650959469054952No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7240.15916040687027766No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7080.15564304981237098No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC6860.15080668385774929No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6820.14992734459327262No Hit
GGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGG6810.14970750977715344No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6750.14838850088043845No Hit
TCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAGCA6690.14706949198372343No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6670.14662982235148508No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6660.14640998753536594No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6650.14619015271924676No Hit
CACCAATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGA6590.14487114382253174No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6530.14355213492581675No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6490.14267279566134008No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6470.14223312602910174No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA6420.1411339519485059No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT6420.1411339519485059No Hit
CTCCAGCGATGGTCAAAGTGACTGTTGTTCCTGAACTGGAGCCAGAGAATCGCCCAGGGATCCCTTGAGGCCTCT6230.1369570904422417No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC6090.13387940301657336No Hit
GCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTT6040.1327802289359775No Hit
CCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCT5980.1314612200392625No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT5980.1314612200392625No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5920.1301422111425475No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5890.12948270669419No Hit
GCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA5840.12838353261359414No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5700.1253058451879258No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5630.12376700147509162No Hit
CACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGACCATCG5520.12134881849778076No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAG5500.12090914886554244No Hit
GTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACA5490.12068931404942326No Hit
GCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGG5480.1204694792333041No Hit
GGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTTGGCAAAGCATCTCCAGAGCAGGTGATCCTGGC5400.11871080070435076No Hit
CTCCACTGCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCC5370.11805129625599324No Hit
CTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATA5290.1162926177270399No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT5240.11519344364644406No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5200.11431410438196739No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG5190.11409426956584823No Hit
GTTCAGGAACAACAGTCACTTTGACCATCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAG5120.11255542585301404No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5070.1114562517724182No Hit
CAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGT5020.11035707769182236No Hit
GGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCT5000.10991740805958401No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC4950.10881823397898818No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4940.10859839916286902No Hit
GCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT4930.10837856434674986No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4920.10815872953063069No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4910.10793889471451151No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4900.10771905989839234No Hit
CCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG4780.10508104210496233No Hit
GGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCC4700.10332236357600899No Hit
CAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGT4670.10266285912765148No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA4620.10156368504705565No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA4560.10024467615034063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)