FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053926
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGGGGTTACTACTGGACCTGGATCCGCCAGCC51870.4921597911048783No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA38770.3678626393124375No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30650.29081738186552No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26240.24897383687279753No Hit
GTTTATTACTGTGCGAGAGGGGGATATTGTAGTGGTGACACCTGCTTCTCACCGCTCGGCTATGACCACTGGGGC21910.20788935845590678No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18600.1764829788808702No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18050.17126439617202727No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17670.16765882993682668No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15880.1506747153025924No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15590.14792309896520248No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15260.14479194933989673No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14400.13663198364970594No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14250.13520873382002152No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13730.13027480107711548No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13710.13008503443315755No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13370.1268590014858728No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCATAGCCGA12810.12154553545505092No Hit
GTATAACAGTGCCCCTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT12750.12097623552317716No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12690.12040693559130337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12220.11594741945829214No Hit
CTACAATATCCCCCTCTCGCACAGTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAA11920.11310091979892327No Hit
GGTCATAGCCGAGCGGTGAGAAGCAGGTGTCACCACTACAATATCCCCCTCTCGCACAGTAATAAACAGCCGTGT11880.11272138651100742No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT11770.11167766996923884No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG11720.111203253359344No Hit
CTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGCTTGGGGAAATCAATCATAGTGGAAACAC11510.1092107035977858No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11460.10873628698789099No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11340.10759768712414344No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGGGGTTACTACTGGA10800.10247398773727946No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10570.10029167133176332No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT10550.10010190468780542No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10550.10010190468780542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)