FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053926
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGGGGTTACTACTGGACCTGGATCCGCCAGCC50450.47868635938386567No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA37460.35543292413319344No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34670.32896047730106287No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26090.24755058704311306No Hit
GTTTATTACTGTGCGAGAGGGGGATATTGTAGTGGTGACACCTGCTTCTCACCGCTCGGCTATGACCACTGGGGC20240.19204384368542005No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18270.17335182925556442No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17370.1648123302774578No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16790.159309097602678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16640.15788584777299355No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16350.15513423143560365No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15640.1483975155750973No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15040.14270451625635955No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14440.1370115169376218No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT13140.12467668508035668No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13110.1243920351144198No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12790.12135576881109301No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCATAGCCGA12340.11708601932203969No Hit
GTATAACAGTGCCCCTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT11860.11253161986704949No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC11740.11139302000330194No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11700.11101348671538609No Hit
CTACAATATCCCCCTCTCGCACAGTAATAAACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAA11490.10902093695382789No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC11440.10854652034393306No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11070.10503583743071146No Hit
GGTCATAGCCGAGCGGTGAGAAGCAGGTGTCACCACTACAATATCCCCCTCTCGCACAGTAATAAACAGCCGTGT11010.10446653749883769No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC10970.10408700421092185No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT10940.10380235424498495No Hit
GTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGGGGTTACTACTGGA10720.10171492116144776No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT10600.10057632129770022No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG10550.10010190468780542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17200.039.1534541
TGGCTTG16700.037.84648570
GTGGTAT3250.037.191921
TATCAAC19800.033.8039132
ATCAACG20800.032.1436043
TCAACGC21100.031.6805784
CAACGCA21250.031.456955
AACGCAG21750.031.0506426
CGCAGAG22350.029.9087318
ACGCAGA22700.029.7511657
ATAGGTG8350.029.2985174
AGAGTAC22600.029.27295711
CAGAGTA23500.028.15186510
CATAGGT8700.028.125183
TAGGTGG8950.027.3343735
TCATAGG9400.026.7932572
GCAGAGT25050.026.5474859
TCAGGTC9550.025.97783312
GTATTAT809.494734E-525.9015141
GTGGCTT17150.025.7293569
Produced by FastQC (version 0.11.5)