FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641811
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG707.2759576E-1249.50649640
CTCGTAT751.6370905E-1146.16619539
GTATCAA18850.043.9680521
CGTATGC856.184564E-1140.80850641
TATCAAC21100.039.7653732
CAACGCA21950.037.912165
ATCAACG22150.037.7250863
TCAACGC22500.037.1382564
AACGCAG22450.037.067796
GGTATCA7800.034.8308451
CGCAGAG24650.033.8990148
AGAGTAC25000.033.42442711
CAGAGTA25550.032.5703310
ACGCAGA26150.031.9545177
GCAGAGT27150.030.6509029
GAGTACG21900.030.30476412
AGTACGG22050.030.25455913
CTACGTT500.006684314528.43582266
TACGGGT4200.027.83731315
CGAATTA500.00785689227.50981715
Produced by FastQC (version 0.11.5)