FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641811
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8200.1277634693079427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20150.040.5479551
GGTATCA8750.038.611231
TATCAAC22700.035.7767942
CAACGCA23200.034.496135
TCAACGC23350.034.2772034
ATCAACG23500.034.2179153
AACGCAG24400.033.2219166
GTGGTAT3350.032.930691
AGAGTAC25900.031.56310511
CGCGCAA450.00413263431.37490762
CGCAGAG25900.031.2978698
ACGCAGA26350.030.7633697
CAGAGTA26450.030.6470610
TGGTATC3850.029.4963652
ATTTCGC500.00682536228.3144964
GCAGAGT28750.028.1952959
AGTACGG22200.027.38563513
GAGTACG22150.027.29238112
TACGGGA7350.026.16996615
GTACGGG23850.025.20299514
Produced by FastQC (version 0.11.5)